Establishment of Rapid Propagation for sterile Seedlings of <i>Anoectochilus emeiensis</i> by Tissue Culture

LU Song; XIONG Tie-yi

doi:10.16779/j.cnki.1003-5508.2017.01.001

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Volume 38 Issue 1

Nov. 2019

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LU Song, XIONG Tie-yi. Establishment of Rapid Propagation for sterile Seedlings of Anoectochilus emeiensis by Tissue Culture[J]. Journal of Sichuan Forestry Science and Technology, 2017, 38(1): 1-5. doi: 10.16779/j.cnki.1003-5508.2017.01.001

Citation:

LU Song, XIONG Tie-yi. Establishment of Rapid Propagation for sterile Seedlings of Anoectochilus emeiensis by Tissue Culture[J]. Journal of Sichuan Forestry Science and Technology, 2017, 38(1): 1-5. doi: 10.16779/j.cnki.1003-5508.2017.01.001

Establishment of Rapid Propagation for sterile Seedlings of Anoectochilus emeiensis by Tissue Culture

doi: 10.16779/j.cnki.1003-5508.2017.01.001

LU Song²,
XIONG Tie-yi^1,2

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Sichuan Natural Resources Science Academy, Chengdu 610015, Sichuan, China;Emei Mountain Biotic Resource Experimental Station, Mount Emei 614201, Sichuan, China

Received Date: 2016-12-21

Abstract

Culture in Vitro and propagation were carried out with capsules of Anoectochilus emeiensis by using orthogonal design with 4 levels of 5 factors.The result showed that the best explants disinfection method was to immerse 30 s in 75% alcohol plus 0.1%HgCl₂;the best medium for primary culture was MS+10% banana juice;MS+10% banana+6-BA 2 mg·L^-1+NAA 0.5 mg·L^-1+AC 3g·L^-1was the most suitable for bud induction and multiplication and the optimum medium for rooting and strong seedling culture was 1/2MS+10% banana+ NAA 2 mg·L^-1+6-BA 0.5 mg·L^-1+ AC 1g·L^-1.
- Anoectochilus emeiensis K.Y.Lang.;
- Seeds;
- Tissue culture;
- asexual propagation
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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Establishment of Rapid Propagation for sterile Seedlings of Anoectochilus emeiensis by Tissue Culture

doi: 10.16779/j.cnki.1003-5508.2017.01.001

LU Song²,
XIONG Tie-yi^1,2

Sichuan Natural Resources Science Academy, Chengdu 610015, Sichuan, China;Emei Mountain Biotic Resource Experimental Station, Mount Emei 614201, Sichuan, China

Received Date: 2016-12-21

Key words:

Abstract: Culture in Vitro and propagation were carried out with capsules of Anoectochilus emeiensis by using orthogonal design with 4 levels of 5 factors.The result showed that the best explants disinfection method was to immerse 30 s in 75% alcohol plus 0.1%HgCl₂;the best medium for primary culture was MS+10% banana juice;MS+10% banana+6-BA 2 mg·L^-1+NAA 0.5 mg·L^-1+AC 3g·L^-1was the most suitable for bud induction and multiplication and the optimum medium for rooting and strong seedling culture was 1/2MS+10% banana+ NAA 2 mg·L^-1+6-BA 0.5 mg·L^-1+ AC 1g·L^-1.