A Rapid Detection Method for the Pathogen of Pine Needle Blight

YUAN Chuan; WU Nan; LIU Ying-gao; XU Xiu-lan; ZHAO Jing-yang; LI Wan-yan

doi:10.16779/j.cnki.1003-5508.2019.03.016

In order to realize the early detection of Pestalotiopsis funerea in Pine needles,the specific primer AF(R/F) was designed,and PCR detection system was established according to the conserved sequence of ITS region of P.funerea.The results showed that the method could amplify a single band of 480 bp in genomic DNA of P.funerea and pine needle genomic DNA. P.funerea could be detected in tissues without obvious symptoms.The established PCR detection system was suitable for molecular identification of P.funerea and early diagnosis of pine needle blight.

A Rapid Detection Method for the Pathogen of Pine Needle Blight

Forestry Technology Promotion Station, Guang'an 638000, China;Sichuan Association for Forest Integrated Pest Management, Chengdu 610081, China;College of Forestry, Sichuan Agricultural University, Chengdu 611130, China

Received Date: 2019-02-19

Key words:

Abstract: In order to realize the early detection of Pestalotiopsis funerea in Pine needles,the specific primer AF(R/F) was designed,and PCR detection system was established according to the conserved sequence of ITS region of P.funerea.The results showed that the method could amplify a single band of 480 bp in genomic DNA of P.funerea and pine needle genomic DNA. P.funerea could be detected in tissues without obvious symptoms.The established PCR detection system was suitable for molecular identification of P.funerea and early diagnosis of pine needle blight.

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Reference (19)

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A Rapid Detection Method for the Pathogen of Pine Needle Blight

doi: 10.16779/j.cnki.1003-5508.2019.03.016

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通讯作者: 陈斌, bchen63@163.com

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