中文
Old Website

WE ARE COMMITTED TO REPORTING THE LATEST FORESTRY ACADEMIC ACHIEVEMENTS

Advanced Search
Volume 40 Issue 2
Nov.  2019
Article Contents
Turn off MathJax
Article Navigation >  Journal of Sichuan Forestry Science and Technology  > 2019  >  40(2): 42-47

LI Jia-man, HUANG Zhen, YANG Han-bo, CHEN Zhi, XING Wen-xi, JIN Wei, GUO Hong-ying. A Study of Tissue Culture of Cinnamomum longepaniculatum[J]. Journal of Sichuan Forestry Science and Technology, 2019, 40(2): 42-47. doi: 10.16779/j.cnki.1003-5508.2019.02.010
Citation: LI Jia-man, HUANG Zhen, YANG Han-bo, CHEN Zhi, XING Wen-xi, JIN Wei, GUO Hong-ying. A Study of Tissue Culture of Cinnamomum longepaniculatum[J]. Journal of Sichuan Forestry Science and Technology, 2019, 40(2): 42-47. doi: 10.16779/j.cnki.1003-5508.2019.02.010
shu

A Study of Tissue Culture of Cinnamomum longepaniculatum


doi: 10.16779/j.cnki.1003-5508.2019.02.010
More Information

  • Sichuan Academy of Forestry, Chengdu 610081, China

  • Received Date: 2018-12-10
  • The tissue culture technique was selected for Cinnamomum longepaniculatum to breed excellent nursery stock, by analyzing and comparing the influence of disinfectant varieties, concentration and action time, MS medium, 6-BA, NAA and IBA on tissue culture of annual shoots. The results showed that contamination rates were no higher than 15% and survival rates were higher than 70%, under 10% of 84 disinfectant treatment for 15min, or 15% of 84 disinfectant treatment for 10 min. The optimum medium for adventitious bud regeneration was sucrose 30 g·L-1+carrageenan 6 g·L-1+MS+6-BA 1.0 mg·L-1+ IBA 0.1 mg·L-1; The medium for multiplication of adventitious buds was sucrose 30 g·L-1+carrageenan 6 g·L-1+MS+6-BA 1.0 mg·L-1+NAA 0.10 mg·L-1+IBA 0.2 mg·L-1; The rooting medium was sucrose 15 g·L-1+carrageenan 6 g·L-1+1/2MS+IBA 1.5 mg·L-1+NAA 1.0 mg·L-1.
  • 加载中
  • [1] 程必强,喻学俭,丁靖垲,等.中国樟属植物资源及其芳香成分[M].昆明:云南科技出版社,1997,34~35.
    [2] 魏琴,李群,罗扬,等.油樟油对植物病原真菌活性的抑制作用[J].中国油料作物学报,2006,28(1):63~66.
    [3] 叶奎川.油樟叶提取物的体外抗肝癌活性及其作用机理研究[D].成都:四川农业大学,2012.
    [4] 陶翠,魏琴,殷中琼,等.油樟叶挥发油对三种真菌的抗菌效果[J].中国兽医科学,2011,41(1):89~93.
    [5] 刘盼,刘燕,陈斯伟,等.气相色谱法测定油樟油中枝油精的含量[J].西南医科大学学报,2017,40(1):64~66.
    [6] 廖时权.宜宾县开发天然油樟[J].农村经济与科技,1995,(8):31.
    [7] 罗中杰,李维一,魏琴,等.宜宾油樟的现状及未来[J].四川师范大学学报:自然科学版,2001,24(3):317~319.
    [8] 李斌,林源,唐军荣,等.无籽刺梨的组织培养研究[J].经济林研究,2016,34(3):142~147.
    [9] 汤雪燕,赵统利,邵小斌,等.植物组织培养的污染防治[J].江苏农业科学,2014,(1):50~52.
    [10] 王曙光,丁雨龙,林树燕,等.慈竹组织培养消毒方法筛选及丛芽诱导研究[J].西部林业科学,2013,(1):38~41.
    [11] 王涌鑫,关宁,李聪.高效的苜蓿组织培养再生体系的建立[J].东北师大学报:自然科学版,2008,40(3):112~117.
    [12] 邹娜,李意,连芳青.优良观赏药用地被植物——白芨组织培养及快速繁殖研究[J].江西农业大学学报,2013,35(5):950~955.
    [13] 周锦霞,周黎军,魏琴.油樟组织培养污染率控制试验[J].宜宾学院学报,2006,(6):33~34.
    [14] 魏琴,周黎军,宣朴,等.组培条件对油樟试管苗玻璃化的影响[J].四川师范大学学报:自然科学版,2006,29(5):606~608.
    [15] 张日清,刘海龙,汪灵丹,等.榉树组培芽继代增殖的影响因素[J].经济林研究,2013,31(3):54~58.
    [16] Dhiman M,Shatma V,Moitra S.Somatic embryogenesis and plant regeneration in Ephedrafoliates(Boiss.);anonconiferous gymnosperm.Plant Tissue Cultureand Biotechnology,2010,20(2):133~143.
    [17] 魏琴,李想韵,王丽,等.不同因素对油樟愈伤组织生长的影响[J].安徽农业科学,2008,36(17):7135~7137.
    [18] 孙敬三,朱至清.植物细胞工程实验技术[M].北京:化学工业出版社,2006:28~55.
    [19] 李浚明.植物组织培养教程[M].朱登云译,北京:中国农业大学出版社,2005:39~64.
    [20] 辛亚龙,唐军荣,杨宇明,等.牛樟组织培养技术研究[J].中南林业科技大学学报,2017,37(8):48~53.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Get Citation
PDF
XML

Article views(368) PDF downloads(10) Cited by()

Related
Proportional views

A Study of Tissue Culture of Cinnamomum longepaniculatum

doi: 10.16779/j.cnki.1003-5508.2019.02.010
  • Sichuan Academy of Forestry, Chengdu 610081, China
  • Received Date: 2018-12-10

Abstract: The tissue culture technique was selected for Cinnamomum longepaniculatum to breed excellent nursery stock, by analyzing and comparing the influence of disinfectant varieties, concentration and action time, MS medium, 6-BA, NAA and IBA on tissue culture of annual shoots. The results showed that contamination rates were no higher than 15% and survival rates were higher than 70%, under 10% of 84 disinfectant treatment for 15min, or 15% of 84 disinfectant treatment for 10 min. The optimum medium for adventitious bud regeneration was sucrose 30 g·L-1+carrageenan 6 g·L-1+MS+6-BA 1.0 mg·L-1+ IBA 0.1 mg·L-1; The medium for multiplication of adventitious buds was sucrose 30 g·L-1+carrageenan 6 g·L-1+MS+6-BA 1.0 mg·L-1+NAA 0.10 mg·L-1+IBA 0.2 mg·L-1; The rooting medium was sucrose 15 g·L-1+carrageenan 6 g·L-1+1/2MS+IBA 1.5 mg·L-1+NAA 1.0 mg·L-1.

    HTML

Reference (20)

Catalog

    Back Issue Browsing

    Wechat

    《四川林业科技》编辑部版权所有 蜀ICP备19031520号-2  ssl证书签章   百度统计

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return