ZHANG Li. Induction of Callus and Adventitious Bud in Larix olgensis[J]. Journal of Sichuan Forestry Science and Technology, 2019, 40(1): 72-76. doi: 10.16779/j.cnki.1003-5508.2019.01.016
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ZHANG Li. Induction of Callus and Adventitious Bud in Larix olgensis[J]. Journal of Sichuan Forestry Science and Technology, 2019, 40(1): 72-76. doi: 10.16779/j.cnki.1003-5508.2019.01.016
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Induction of Callus and Adventitious Bud in Larix olgensis
More Information
- Received Date: 2018-11-14
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Abstract
The mature zygotic embryo of Larix olgensis was used as the research object.The callus and adventitious bud induction experiments were made to select disinfection method of explants, appropriate medium and hormone combination for L.olgensis.The optimum disinfection method was to sterilize peeled seeds with 4% NaClO for 15 min,and to disinfect the embryos with 0.5% NaClO for 2 min;BM was the optimum medium for callus induction;In BM medium supplemented with 2.0 mg·l-1 TDZ,the callus induction reached the highest rate,89%;In BM medium combined with 1.5 mg·l-1 TDZ,the adventitious bud induction reached the highest rate,80.0%.
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References
|
[1]
|
杨传平.长白落叶松种群遗传变异与利用[M].黑龙江:东北林业大学出版社,2001:11~20. |
|
[2]
|
徐悦丽.长白落叶松群体遗传变异及4CL基因单核苷酸多态性的研究[D].黑龙江:东北林业大学,2012:1~2. |
|
[3]
|
Bonga J M,Pond S E.Adventitious shoot formation in cultures of 30-yeaer-old Larix decidua,Larix leptolepis,Larix xeurolepis and Larix laricina trees[J].Plant Cell Tissue and Organ Culture,1991,26(1):45~51. |
|
[4]
|
齐力旺,韩素英,杨云龙,等.华北落叶松的组织培养再生植株[J].植物生理学通讯,1996,32(2):128~129. |
|
[5]
|
Lin X F,Zhang W B,Takano H,et al.Efficient plant regeneration and micropropagation from callus derived from mature zygotic embryo of Larix gmelinii[J].Plant Biotechnology,2004,21(2):159~163. |
|
[6]
|
Chesick E E,Bilderback D E,Blake G M.In vitro multiple bud formation by 20-year-old westerm larch buds and stems[J].Hort Science,1990,25(1):114~116. |
|
[7]
|
范岳霞,袁文华,孔冬梅.彰武松腋芽诱导研究[J].中国农学通报,2013,29(16):7~11. |
|
[8]
|
赵健,李旦,张金凤.华北落叶松成熟合子胚不定芽诱导及伸长研究[J].中国农学通报,2014,30(16):12~17. |
|
[9]
|
唐巍,欧阳藩,郭仲琛.火炬松成熟合子胚培养直接器官发生和植株再生[J].云南植物研究,1997,19(3):285~288. |
|
[10]
|
王伟达,李成浩,张含国,等.长白落叶松愈伤组织诱导与不定芽分化[J].东北林业大学学报,2008,36(2):6~7. |
|
[11]
|
朱晓丹,李桐森,邓莉兰.云南松成熟胚的离体培养[J].贵州林业科技,2005,33(4):29~30. |
|
[12]
|
曹焱,孟庆峰,刁秋实,等.植物生长调节物质对成熟红松合子胚愈伤组织诱导的影响[J].中国林副特产,2015,4(3):10~12. |
|
[13]
|
秦静远,黄玉敏.杜鹃的组织培养及快速繁殖[J].植物生理学通讯,2003,39(1):38. |
|
[14]
|
Ibrahim R.and Debergh P C.2000.Improvement of adventitious bud formantion and plantlet regeneration from in vitro leaflet explants of roses (Rosa hybrida L.) Acta Hort.520 ISHS.271~276. |
|
[15]
|
Preece J E,Huetteman C A,Ashby W C.Micto and cutting propagation of silver maple.I.Results with adult and juvenile propagules.Jam Soc Hortic,Sci,1991,116:142~148. |
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