Physiological and Biochemical Responses of Forest Tree Seedlings of 4 Species at the Early Growth Stage under Heavy Metal Cadmium (Cd) Stress

LUO Yan; LI Yu-dong; YAO Min; LUO Xiao-bo; WANG Qiong-yao

doi:10.16779/j.cnki.1003-5508.2018.05.020

Volume 39 Issue 5

Nov. 2019

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Article Navigation > Journal of Sichuan Forestry Science and Technology > 2018 > 39(5): 87-92

LUO Yan, LI Yu-dong, YAO Min, LUO Xiao-bo, WANG Qiong-yao. Physiological and Biochemical Responses of Forest Tree Seedlings of 4 Species at the Early Growth Stage under Heavy Metal Cadmium (Cd) Stress[J]. Journal of Sichuan Forestry Science and Technology, 2018, 39(5): 87-92. doi: 10.16779/j.cnki.1003-5508.2018.05.020

Citation:

LUO Yan, LI Yu-dong, YAO Min, LUO Xiao-bo, WANG Qiong-yao. Physiological and Biochemical Responses of Forest Tree Seedlings of 4 Species at the Early Growth Stage under Heavy Metal Cadmium (Cd) Stress[J]. Journal of Sichuan Forestry Science and Technology, 2018, 39(5): 87-92. doi: 10.16779/j.cnki.1003-5508.2018.05.020

Physiological and Biochemical Responses of Forest Tree Seedlings of 4 Species at the Early Growth Stage under Heavy Metal Cadmium (Cd) Stress

doi: 10.16779/j.cnki.1003-5508.2018.05.020

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Sichuan Provincial Academy of Natural Resource Sciences, Chengdu 610024, China;Emei Mountain Resource Experimental Station, Mount Emei 614201, China;College of Life Sciences Sichuan University, Chengdu 610064, China

Received Date: 2018-07-02

Abstract

In order to study the response mechanism in the early stage of seedling growth under Cd stress,the changes of physiological and biochemical indexes were analyzed,which included external morphology,activities of superoxide dismutase (SOD),Peroxidase(POD) catalase (CAT),contents of glutathione (GSH),malondialdehyde (MDA) and chlorophyll (CHL) in 4 kinds of native woody plants(Acer pentaphyllum, Toona ciliata, Alnus cremastogyne, Pinus yunnanensis).The results were showed as below.In terms of external morphology,the underground length of Acer pentaphyllum and Alnus cremastogyne decreased significantly,and the upper part of T.ciliata dropped significantly,and there was no significant change for the ground and underground parts of P.yunnanensis.In SOD activity,Acer pentaphyllum, T.ciliata, Alnus cremastogyne and P.yunnanensis increased significantly with the increase of Cd concentration.As for CAT activity,Acer pentaphyllum, T.ciliata and P.yunnanensis increased significantly with the increase of Cd concentration,while Alnus cremastogyne was significantly higher than that of control group only when Cd concentration was 100 mg·kg^-1.In POD activity,Acer pentaphyllum, T.ciliata, Alnus cremastogyne and P.yunnanensis increased significantly with the increase of Cd concentration.On the GSH side,with the increase of Cd concentration,the contents of Acer pentaphyllum and P.yunnanensis decreased significantly,and T.ciliata and Alnus cremastogyne were not significantly reduced.In terms of MDA and CHL content,these 4 kinds of forest trees showed a significant decrease in high concentration Cd stress,but varied in low concentration.In general,under the stress of cadmium,the seedlings of these 4 kinds of trees changed significantly in terms of physiology and ecology,and showed good tolerance in low concentration.
- Cadmium stress;
- Forest trees;
- Seedling;
- Antioxidant system

References

[1]	刘翠华,依艳丽,张大庚,等.葫芦岛锌厂周围土壤镉污染现状研究[J].土壤通报,2003,34(4):326~329.
[2]	刘玉机,房聚燕,刘绮等.辽宁省环境重金属研究[M].北京:中国环境科学出版社,1996.
[3]
[4]
[5]	向涛.草本花卉对镉污染土壤修复研究[D]; 重庆大学,2014.
[6]	陈良华,徐睿,杨万勤,等.镉污染条件下香樟和油樟对镉的吸收能力和耐性差异[J].生态环境学报,2015,2):316~322.
[7]	黄会一,蒋德明.木本植物对土壤中镉的吸收,积累和耐性[J].中国环境科学,1989,9(5):323~330.
[8]	李橙.利用绿化苗木修复Cd污染土壤研究[D]; 河北农业大学,2011.
[9]	李金花,何燕,段建平,等.107杨对土壤重金属的吸收和富集[J].林业科学研究,2012,25(1):65~70.
[10]	杨园,王艮梅.杨树对镉胁迫的响应及抗性机制研究进展[J].世界林业研究,2017,30(4):29~34.
[11]	罗艳,李裕冬,罗晓波,等.重金属镉对4种林木种子萌发及幼苗生长的影响[J].四川林业科技,2018,39(2):7~12.
[12]	吴永波,叶波.高温干旱复合胁迫对构树幼苗抗氧化酶活性和活性氧代谢的影响[J].生态学报,2016,36(2):403~410.
[13]	Low P,Merida J.The oxidative burst in plant defense:Function and signal transduction[J].Physiologia Plantarum,2010,96(3):533~542.
[14]	刘家忠,龚明.植物抗氧化系统研究进展[J].云南师范大学学报:自然科学版,1999,6):1~11.
[15]	陈少裕.植物谷胱甘肽的生理作用及其意义[J].植物生理学通讯,1993,29(3):210~214.
[16]	高俊凤.植物生理学实验指导[M].高等教育出版社,2006.
[17]	赵世杰,史国安,董新纯.植物生理学实验指导[M].北京:中国农业科学技术出版社,2002.
[18]	熊庆娥.植物生理学实验教程[M].四川出版集团,2003.
[19]	Younis M,Hasaneen M,Tourky S.Plant growth,metabolism and adaptation in relation to stress conditions.XXIV.Salinity-biofertility interactive effects on proline,glycine and various antioxidants in Lactuca sativa[J].Protoplasma,2009,2(5):197~205.
[20]	邓旭,王娟,谭济才.外来入侵种豚草对不同环境胁迫的生理响应[J].植物生理学报,2010,46(10):1013~9.
[21]	王敬华,张立芳,施国新,等.镍添加对水鳖(Hydrocharis dubia)叶片镍和营养元素含量以及氧化胁迫和抗氧化系统的影响[J].湖泊科学,2016,28(3):599~608.
[22]	Prashanth S,Sadhasivam V,Parida A.Over expression of cytosolic copper/zinc superoxide dismutase from a mangrove plant Avicennia marina in indica rice var Pusa Basmati-1 confers abiotic stress tolerance[J].Transgenic Research,2008,17(2):281~91.
[23]	谢亚军,王兵,梁新华,等.干旱胁迫对甘草幼苗活性氧代谢及保护酶活性的影响[J].农业科学研究,2008,29(4):19~22.
[24]	陈少裕.膜脂过氧化与植物逆境胁迫[J].植物学报,1989,6(4):211~217.
[25]	田治国,王飞.不同品种万寿菊对镉胁迫的生长和生理响应[J].西北植物学报,2013,33(10):2057~2064.
[26]	蒋明义,杨文英,徐江,等.渗透胁迫诱导水稻幼苗的氧化伤害[J].作物学报,1994,(6):733~738.
[27]	刘柿良,杨容孑,马明东,等.土壤镉胁迫对龙葵(Solanum nigrum L.)幼苗生长及生理特性的影响[J].农业环境科学学报,2015,(2):240~247.
[28]	苏明洁,廖源林,叶充,等.镉胁迫下苦楝(Melia azedarach L.)幼苗的生长及生理响应[J].农业环境科学学报,2016,35(11):2086~2093.
[29]	简敏菲,杨叶萍,余厚平,等.不同浓度Cd~(2+)胁迫对苎麻叶绿素及其光合荧光特性的影响[J].植物生理学报,2015,(8):1331~1338.
[30]	贾中民,王力,魏虹,等.垂柳和旱柳对镉的积累及生长光合响应比较分析[J].林业科学,2013,49(11):51~59.

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Physiological and Biochemical Responses of Forest Tree Seedlings of 4 Species at the Early Growth Stage under Heavy Metal Cadmium (Cd) Stress

Sichuan Provincial Academy of Natural Resource Sciences, Chengdu 610024, China;Emei Mountain Resource Experimental Station, Mount Emei 614201, China;College of Life Sciences Sichuan University, Chengdu 610064, China

Received Date: 2018-07-02

Key words:

Abstract: In order to study the response mechanism in the early stage of seedling growth under Cd stress,the changes of physiological and biochemical indexes were analyzed,which included external morphology,activities of superoxide dismutase (SOD),Peroxidase(POD) catalase (CAT),contents of glutathione (GSH),malondialdehyde (MDA) and chlorophyll (CHL) in 4 kinds of native woody plants(Acer pentaphyllum, Toona ciliata, Alnus cremastogyne, Pinus yunnanensis).The results were showed as below.In terms of external morphology,the underground length of Acer pentaphyllum and Alnus cremastogyne decreased significantly,and the upper part of T.ciliata dropped significantly,and there was no significant change for the ground and underground parts of P.yunnanensis.In SOD activity,Acer pentaphyllum, T.ciliata, Alnus cremastogyne and P.yunnanensis increased significantly with the increase of Cd concentration.As for CAT activity,Acer pentaphyllum, T.ciliata and P.yunnanensis increased significantly with the increase of Cd concentration,while Alnus cremastogyne was significantly higher than that of control group only when Cd concentration was 100 mg·kg^-1.In POD activity,Acer pentaphyllum, T.ciliata, Alnus cremastogyne and P.yunnanensis increased significantly with the increase of Cd concentration.On the GSH side,with the increase of Cd concentration,the contents of Acer pentaphyllum and P.yunnanensis decreased significantly,and T.ciliata and Alnus cremastogyne were not significantly reduced.In terms of MDA and CHL content,these 4 kinds of forest trees showed a significant decrease in high concentration Cd stress,but varied in low concentration.In general,under the stress of cadmium,the seedlings of these 4 kinds of trees changed significantly in terms of physiology and ecology,and showed good tolerance in low concentration.

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Reference (30)

[1]	刘翠华,依艳丽,张大庚,等.葫芦岛锌厂周围土壤镉污染现状研究[J].土壤通报,2003,34(4):326~329.
[2]	刘玉机,房聚燕,刘绮等.辽宁省环境重金属研究[M].北京:中国环境科学出版社,1996.
[3]
[4]
[5]	向涛.草本花卉对镉污染土壤修复研究[D]; 重庆大学,2014.
[6]	陈良华,徐睿,杨万勤,等.镉污染条件下香樟和油樟对镉的吸收能力和耐性差异[J].生态环境学报,2015,2):316~322.
[7]	黄会一,蒋德明.木本植物对土壤中镉的吸收,积累和耐性[J].中国环境科学,1989,9(5):323~330.
[8]	李橙.利用绿化苗木修复Cd污染土壤研究[D]; 河北农业大学,2011.
[9]	李金花,何燕,段建平,等.107杨对土壤重金属的吸收和富集[J].林业科学研究,2012,25(1):65~70.
[10]	杨园,王艮梅.杨树对镉胁迫的响应及抗性机制研究进展[J].世界林业研究,2017,30(4):29~34.
[11]	罗艳,李裕冬,罗晓波,等.重金属镉对4种林木种子萌发及幼苗生长的影响[J].四川林业科技,2018,39(2):7~12.
[12]	吴永波,叶波.高温干旱复合胁迫对构树幼苗抗氧化酶活性和活性氧代谢的影响[J].生态学报,2016,36(2):403~410.
[13]	Low P,Merida J.The oxidative burst in plant defense:Function and signal transduction[J].Physiologia Plantarum,2010,96(3):533~542.
[14]	刘家忠,龚明.植物抗氧化系统研究进展[J].云南师范大学学报:自然科学版,1999,6):1~11.
[15]	陈少裕.植物谷胱甘肽的生理作用及其意义[J].植物生理学通讯,1993,29(3):210~214.
[16]	高俊凤.植物生理学实验指导[M].高等教育出版社,2006.
[17]	赵世杰,史国安,董新纯.植物生理学实验指导[M].北京:中国农业科学技术出版社,2002.
[18]	熊庆娥.植物生理学实验教程[M].四川出版集团,2003.
[19]	Younis M,Hasaneen M,Tourky S.Plant growth,metabolism and adaptation in relation to stress conditions.XXIV.Salinity-biofertility interactive effects on proline,glycine and various antioxidants in Lactuca sativa[J].Protoplasma,2009,2(5):197~205.
[20]	邓旭,王娟,谭济才.外来入侵种豚草对不同环境胁迫的生理响应[J].植物生理学报,2010,46(10):1013~9.
[21]	王敬华,张立芳,施国新,等.镍添加对水鳖(Hydrocharis dubia)叶片镍和营养元素含量以及氧化胁迫和抗氧化系统的影响[J].湖泊科学,2016,28(3):599~608.
[22]	Prashanth S,Sadhasivam V,Parida A.Over expression of cytosolic copper/zinc superoxide dismutase from a mangrove plant Avicennia marina in indica rice var Pusa Basmati-1 confers abiotic stress tolerance[J].Transgenic Research,2008,17(2):281~91.
[23]	谢亚军,王兵,梁新华,等.干旱胁迫对甘草幼苗活性氧代谢及保护酶活性的影响[J].农业科学研究,2008,29(4):19~22.
[24]	陈少裕.膜脂过氧化与植物逆境胁迫[J].植物学报,1989,6(4):211~217.
[25]	田治国,王飞.不同品种万寿菊对镉胁迫的生长和生理响应[J].西北植物学报,2013,33(10):2057~2064.
[26]	蒋明义,杨文英,徐江,等.渗透胁迫诱导水稻幼苗的氧化伤害[J].作物学报,1994,(6):733~738.
[27]	刘柿良,杨容孑,马明东,等.土壤镉胁迫对龙葵(Solanum nigrum L.)幼苗生长及生理特性的影响[J].农业环境科学学报,2015,(2):240~247.
[28]	苏明洁,廖源林,叶充,等.镉胁迫下苦楝(Melia azedarach L.)幼苗的生长及生理响应[J].农业环境科学学报,2016,35(11):2086~2093.
[29]	简敏菲,杨叶萍,余厚平,等.不同浓度Cd~(2+)胁迫对苎麻叶绿素及其光合荧光特性的影响[J].植物生理学报,2015,(8):1331~1338.
[30]	贾中民,王力,魏虹,等.垂柳和旱柳对镉的积累及生长光合响应比较分析[J].林业科学,2013,49(11):51~59.

Physiological and Biochemical Responses of Forest Tree Seedlings of 4 Species at the Early Growth Stage under Heavy Metal Cadmium (Cd) Stress

doi: 10.16779/j.cnki.1003-5508.2018.05.020

Abstract

References

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通讯作者: 陈斌, bchen63@163.com

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