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ZHAO Xin-wen. A Study of Inducing 2n Pollen of Populus deltoid by Colchicines[J]. Journal of Sichuan Forestry Science and Technology, 2018, 39(4): 37-40. doi: 10.16779/j.cnki.1003-5508.2018.04.009
Citation: ZHAO Xin-wen. A Study of Inducing 2n Pollen of Populus deltoid by Colchicines[J]. Journal of Sichuan Forestry Science and Technology, 2018, 39(4): 37-40. doi: 10.16779/j.cnki.1003-5508.2018.04.009

A Study of Inducing 2n Pollen of Populus deltoid by Colchicines


doi: 10.16779/j.cnki.1003-5508.2018.04.009
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  • Received Date: 2018-04-17
  • In order to get the triploids of P.deltoid,the technique was researched to induce 2n pollen of P.deltoid by colchicines by two-factor randomized block design.The results indicated that(1)a certain proportion of 2n pollen grains could be gotten from the male buds induced by cochicine solution with the concentration of 0.3%,0.5% and 0.8%,under the condition of water culture for 3,4 and 5 days.(2)the proportion of 2n pollen grains could be the highest when the male buds were treated by the cochicine solution of 0.5% under the condition of water culture for 3 days,the percentage of A4(d≧50) could be up to 1.4%,the percentage of 2n pollen (d≧37.5) could be up to 23.4%.
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A Study of Inducing 2n Pollen of Populus deltoid by Colchicines

doi: 10.16779/j.cnki.1003-5508.2018.04.009
  • Liaoning Provincial Institute of Poplar, Gaizhou 115213, China

Abstract: In order to get the triploids of P.deltoid,the technique was researched to induce 2n pollen of P.deltoid by colchicines by two-factor randomized block design.The results indicated that(1)a certain proportion of 2n pollen grains could be gotten from the male buds induced by cochicine solution with the concentration of 0.3%,0.5% and 0.8%,under the condition of water culture for 3,4 and 5 days.(2)the proportion of 2n pollen grains could be the highest when the male buds were treated by the cochicine solution of 0.5% under the condition of water culture for 3 days,the percentage of A4(d≧50) could be up to 1.4%,the percentage of 2n pollen (d≧37.5) could be up to 23.4%.

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