LI Shi-juan, SONG Zu-wen, WANG Li, HUANG Zhong-hua, SHENG Yu-zhen, JIN Kai-ming, ZHANG Ling, XU Lei. Selection of Rhizosphere Phosphate-solubilizing Bacteria for Juglans regia[J]. Journal of Sichuan Forestry Science and Technology, 2018, 39(4): 22-25,53. doi: 10.16779/j.cnki.1003-5508.2018.04.005
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LI Shi-juan, SONG Zu-wen, WANG Li, HUANG Zhong-hua, SHENG Yu-zhen, JIN Kai-ming, ZHANG Ling, XU Lei. Selection of Rhizosphere Phosphate-solubilizing Bacteria for Juglans regia[J]. Journal of Sichuan Forestry Science and Technology, 2018, 39(4): 22-25,53. doi: 10.16779/j.cnki.1003-5508.2018.04.005
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Selection of Rhizosphere Phosphate-solubilizing Bacteria for Juglans regia
More Information
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Sichuan Academy of forestry, Chengdu 610081, China;Forestry Bureau of Xide County, Xide 616750, China;Sichuan Forestrg Propagonda Center Chengdu 61000, China
- Received Date: 2018-04-08
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Abstract
It aimed to gain highly efficient phosphate-solubilizing microorganisms.Phosphate-solubilizing bacteria were isolated in advantage of Pikovskava phosphate dissolving medium.Liquid fermentation experiments showed the phosphate-solubilizing activity,molecular biological methods identified the microbial functional community with higher activity.By single factor test,the vitality of different strains were compared under different formulas and the fermentation conditions of culture medium.Bacillus sp.was the phosphate-solubilizing bacterium with higher efficiency.The optimum medium was as follows:suitable initial pH of 5~9,optimum temperature range of 16℃~40℃,ammonium sulphate,ammonium chloride and ammonium oxalate,peptone for suitable nitrogen source,ammonium oxalate,sucrose,glycerol for suitable carbon source.High concentration of Fe3+ and Mn2+ had a certain role in promoting its vitality,but Cu2+,Ni2+ and Zn2+ would hold the phosphate solubilizing.In different formulations and fermentation conditions,Its phosphate solubilizing ability was better than that of microbial fertilizer 2.In different fermentation conditions,the stability of phosphate solubilizing ability was better than that of microbial fertilizer 1.It would give a certain market potential for the phosphate-solubilizing bacteria added to the fertilizer.
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