A Method of Sex-determination Tests of Giant Pandas

ZHOU Sha; PENG Hua; LIU Jian; HU Jin-yao; YU Ling-fan; MAO Bo-yong

doi:10.16779/j.cnki.1003-5508.2016.06.018

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Volume 37 Issue 6

Nov. 2019

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Article Navigation > Journal of Sichuan Forestry Science and Technology > 2016 > 37(6): 82-85

ZHOU Sha, PENG Hua, LIU Jian, HU Jin-yao, YU Ling-fan, MAO Bo-yong. A Method of Sex-determination Tests of Giant Pandas[J]. Journal of Sichuan Forestry Science and Technology, 2016, 37(6): 82-85. doi: 10.16779/j.cnki.1003-5508.2016.06.018

Citation:

ZHOU Sha, PENG Hua, LIU Jian, HU Jin-yao, YU Ling-fan, MAO Bo-yong. A Method of Sex-determination Tests of Giant Pandas[J]. Journal of Sichuan Forestry Science and Technology, 2016, 37(6): 82-85. doi: 10.16779/j.cnki.1003-5508.2016.06.018

A Method of Sex-determination Tests of Giant Pandas

doi: 10.16779/j.cnki.1003-5508.2016.06.018

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College of Life Science & Biotechnology, Mianyang Normal University, Mianyang 621000, China;Sichuan Academy of Forestry, Chengdu 610081, ;Tian Quan Forestry Bureau in Sichuan Province, Tianquan 625500, China

Received Date: 2016-09-07

Abstract

In this paper,SDS and nucleic acid adsorption column were used for extraction of the giant panda fecal DNA.According to the giant panda sex determination gene primers design factor,BDNF was taken as the positive control.Validation of the effectiveness of the primers was conducted for the use of fecal DNA from artificially reared giant pandas.It was showed that the results were reliable for the identification of the primers.Experiments showed that this method was convenient and reliable.
- Panda;
- DNA extraction;
- Determination of sex
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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A Method of Sex-determination Tests of Giant Pandas

doi: 10.16779/j.cnki.1003-5508.2016.06.018

College of Life Science & Biotechnology, Mianyang Normal University, Mianyang 621000, China;Sichuan Academy of Forestry, Chengdu 610081, ;Tian Quan Forestry Bureau in Sichuan Province, Tianquan 625500, China

Received Date: 2016-09-07

Key words:

Abstract: In this paper,SDS and nucleic acid adsorption column were used for extraction of the giant panda fecal DNA.According to the giant panda sex determination gene primers design factor,BDNF was taken as the positive control.Validation of the effectiveness of the primers was conducted for the use of fecal DNA from artificially reared giant pandas.It was showed that the results were reliable for the identification of the primers.Experiments showed that this method was convenient and reliable.