A Study of the Method of Isolated Culture of <i>Cystoathyrium chinensis</i> Spores

CAI Yu; YU Ling-fan; WEN Zhi-you; GONG Wei; YANG Hong-zhong; ZHAO Chang-ping

doi:10.16779/j.cnki.1003-5508.2016.06.016

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Volume 37 Issue 6

Nov. 2019

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Article Navigation > Journal of Sichuan Forestry Science and Technology > 2016 > 37(6): 76-79

CAI Yu, YU Ling-fan, WEN Zhi-you, GONG Wei, YANG Hong-zhong, ZHAO Chang-ping. A Study of the Method of Isolated Culture of Cystoathyrium chinensis Spores[J]. Journal of Sichuan Forestry Science and Technology, 2016, 37(6): 76-79. doi: 10.16779/j.cnki.1003-5508.2016.06.016

Citation:

CAI Yu, YU Ling-fan, WEN Zhi-you, GONG Wei, YANG Hong-zhong, ZHAO Chang-ping. A Study of the Method of Isolated Culture of Cystoathyrium chinensis Spores[J]. Journal of Sichuan Forestry Science and Technology, 2016, 37(6): 76-79. doi: 10.16779/j.cnki.1003-5508.2016.06.016

A Study of the Method of Isolated Culture of Cystoathyrium chinensis Spores

doi: 10.16779/j.cnki.1003-5508.2016.06.016

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College of Forestry, Sichuan Agricultural University, Chengdu 611134;Sichuan Academy of Forestry, Chengdu 610066;Forestry Bureau of Tianquan, Tianquan 205500

Received Date: 2016-08-08

Abstract

In recent years,the growth environment of Cystoathyrium chinensis Ching has been seriously affected because of the destruction of human interference and geological disaster activities.The species is on the verge of extinction.For the protection and propagation of it,tissue culture propagation techniques were used to turn mature spores as explants.Researches were conducted on influences of spore germination by use of different sterilization,the medium and sucrose concentration.The results showed that the best sterilization was spores through 4 000 r·min^-1 centrifugal prepared spore suspension,0.1% mercuric chloride solution was centrifuged for 5 min,rinsed with sterile water for 4~5 times; the best germination medium was 1/2MS+20 g·L^-1 sucrose.After 90 days,the germination rate could reach 57.8%.
- Cystoathyrium chinensis Ching;
- Spore;
- Germination
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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A Study of the Method of Isolated Culture of Cystoathyrium chinensis Spores

doi: 10.16779/j.cnki.1003-5508.2016.06.016

College of Forestry, Sichuan Agricultural University, Chengdu 611134;Sichuan Academy of Forestry, Chengdu 610066;Forestry Bureau of Tianquan, Tianquan 205500

Received Date: 2016-08-08

Key words:

Abstract: In recent years,the growth environment of Cystoathyrium chinensis Ching has been seriously affected because of the destruction of human interference and geological disaster activities.The species is on the verge of extinction.For the protection and propagation of it,tissue culture propagation techniques were used to turn mature spores as explants.Researches were conducted on influences of spore germination by use of different sterilization,the medium and sucrose concentration.The results showed that the best sterilization was spores through 4 000 r·min^-1 centrifugal prepared spore suspension,0.1% mercuric chloride solution was centrifuged for 5 min,rinsed with sterile water for 4~5 times; the best germination medium was 1/2MS+20 g·L^-1 sucrose.After 90 days,the germination rate could reach 57.8%.