A study of Culture in Vitro and Propagation of Superior Clones of <i>Cunninghamia lanceolata</i>

LIU Hai-ying; WAN Xue-qin; LIU Jun-li; YANG Xiao-rong

doi:10.16779/j.cnki.1003-5508.2016.05.001

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Volume 37 Issue 5

Nov. 2019

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Article Navigation > Journal of Sichuan Forestry Science and Technology > 2016 > 37(5): 1-6

LIU Hai-ying, WAN Xue-qin, LIU Jun-li, YANG Xiao-rong. A study of Culture in Vitro and Propagation of Superior Clones of Cunninghamia lanceolata[J]. Journal of Sichuan Forestry Science and Technology, 2016, 37(5): 1-6. doi: 10.16779/j.cnki.1003-5508.2016.05.001

Citation:

LIU Hai-ying, WAN Xue-qin, LIU Jun-li, YANG Xiao-rong. A study of Culture in Vitro and Propagation of Superior Clones of Cunninghamia lanceolata[J]. Journal of Sichuan Forestry Science and Technology, 2016, 37(5): 1-6. doi: 10.16779/j.cnki.1003-5508.2016.05.001

A study of Culture in Vitro and Propagation of Superior Clones of Cunninghamia lanceolata

doi: 10.16779/j.cnki.1003-5508.2016.05.001

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Department of Forestry, Sichuan Aguicultural University, Ya'an 625014, China;Sichuan Acadamy of Forestry, Chengdu 610081, China

Received Date: 2016-06-12

Abstract

Culture in Vitro and propagation were carried out by using basal sprouts of superior clones 3-3 Cunninghamia lanceolata as explants. The result showed that the optimal cutting trunk height was 30 cm in March and the average number of bud was 47.2; Best explants disinfection solution was to immerse 15s in 70% alcohol and use sterile water to wash twice and then to soak 6 min in 0.1% mercuric chloride and wash 6 times with sterile water; MS culture medium was the optimal basic culture in primary culture; Subculture medium was MS+BA(0.5 mg·L^-1~0.7 mg·L^-1)+IBA(0 mg·L^-1~0.2 mg·L^-1); Inducing-root medium was 1/2MS~1/4MS+0.5 mg·L^-1~1.0 mg·L^-1IBA+0.1 mg·L^-1NAA; The most suitable soil matrix proportion of transplanting was peat soil:yellow subsoil:vermiculite=4:2:1.
- Cunninghamia lanceolata Hook;
- Superior clones;
- Sprout promoting;
- Tissue culture
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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A study of Culture in Vitro and Propagation of Superior Clones of Cunninghamia lanceolata

doi: 10.16779/j.cnki.1003-5508.2016.05.001

Department of Forestry, Sichuan Aguicultural University, Ya'an 625014, China;Sichuan Acadamy of Forestry, Chengdu 610081, China

Received Date: 2016-06-12

Key words:

Abstract: Culture in Vitro and propagation were carried out by using basal sprouts of superior clones 3-3 Cunninghamia lanceolata as explants. The result showed that the optimal cutting trunk height was 30 cm in March and the average number of bud was 47.2; Best explants disinfection solution was to immerse 15s in 70% alcohol and use sterile water to wash twice and then to soak 6 min in 0.1% mercuric chloride and wash 6 times with sterile water; MS culture medium was the optimal basic culture in primary culture; Subculture medium was MS+BA(0.5 mg·L^-1~0.7 mg·L^-1)+IBA(0 mg·L^-1~0.2 mg·L^-1); Inducing-root medium was 1/2MS~1/4MS+0.5 mg·L^-1~1.0 mg·L^-1IBA+0.1 mg·L^-1NAA; The most suitable soil matrix proportion of transplanting was peat soil:yellow subsoil:vermiculite=4:2:1.