A Study of Tissue Culture of <i>Eucalyptus saligna</i>

LIU Jun-li; LIU Hai-ying; LONG Han-li; WU Zong-xing; WU Hua-wei; YANG Xiao-rong

doi:10.16779/j.cnki.1003-5508.2016.04.016

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Volume 37 Issue 4

Nov. 2019

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Article Navigation > Journal of Sichuan Forestry Science and Technology > 2016 > 37(4): 74-78

LIU Jun-li, LIU Hai-ying, LONG Han-li, WU Zong-xing, WU Hua-wei, YANG Xiao-rong. A Study of Tissue Culture of Eucalyptus saligna[J]. Journal of Sichuan Forestry Science and Technology, 2016, 37(4): 74-78. doi: 10.16779/j.cnki.1003-5508.2016.04.016

Citation:

LIU Jun-li, LIU Hai-ying, LONG Han-li, WU Zong-xing, WU Hua-wei, YANG Xiao-rong. A Study of Tissue Culture of Eucalyptus saligna[J]. Journal of Sichuan Forestry Science and Technology, 2016, 37(4): 74-78. doi: 10.16779/j.cnki.1003-5508.2016.04.016

A Study of Tissue Culture of Eucalyptus saligna

doi: 10.16779/j.cnki.1003-5508.2016.04.016

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Sichuan Academy of Forestry, Chengdu 610081, China

Received Date: 2016-04-10

Abstract

Studies were made of tissue culture and fast propagation system of Eucalyptus saligna by taking stem segments with auxiliary buds from superior plants' shooting as explants.The results showed that cutting the superior plants at the trunk base could get the best effect of promoting sprouting with germination rate of 100% and average number of 38 shoots.Late March was the best time to obtain explants and semi-lignified shoots were the best choice.The optimal medium for primary induction was MS+6-BA0.5 mg·L^-1+NAA0.05 mg·L^-1 with a germination rate of 66.67%.The optimal medium for subculture was modified MS+6-BA1.0 mg·L^-1+IBA0.02 mg·L^-1+NAA0.05 mg·L^-1 with a multiplication coefficient of 5.44.The optimal medium for rooting was modified MS+6-BA0.01 mg·L^-1+IBA0.5 mg·L^-1+NAA0.2 mg·L^-1 with a rooting rate of 95% and the average root number of above 9.
- Eucalyptus saligna;
- Promoting sprouting of superior plants;
- Tissue culture;
- Rapid propagation
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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A Study of Tissue Culture of Eucalyptus saligna

doi: 10.16779/j.cnki.1003-5508.2016.04.016

Sichuan Academy of Forestry, Chengdu 610081, China

Received Date: 2016-04-10

Key words:

Eucalyptus saligna /
Promoting sprouting of superior plants /
Tissue culture /
Rapid propagation

Abstract: Studies were made of tissue culture and fast propagation system of Eucalyptus saligna by taking stem segments with auxiliary buds from superior plants' shooting as explants.The results showed that cutting the superior plants at the trunk base could get the best effect of promoting sprouting with germination rate of 100% and average number of 38 shoots.Late March was the best time to obtain explants and semi-lignified shoots were the best choice.The optimal medium for primary induction was MS+6-BA0.5 mg·L^-1+NAA0.05 mg·L^-1 with a germination rate of 66.67%.The optimal medium for subculture was modified MS+6-BA1.0 mg·L^-1+IBA0.02 mg·L^-1+NAA0.05 mg·L^-1 with a multiplication coefficient of 5.44.The optimal medium for rooting was modified MS+6-BA0.01 mg·L^-1+IBA0.5 mg·L^-1+NAA0.2 mg·L^-1 with a rooting rate of 95% and the average root number of above 9.