Comparison between Albescent and Normal Seedings of <i>Phoebe zhennan</i> by AFLP

ZHANG Wei; CHEN Zhong; LONG Han-li; ZHUANG Guo-qing; LI Xiao-qing

doi:10.16779/j.cnki.1003-5508.2014.04.002

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Volume 35 Issue 4

Nov. 2019

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Article Navigation > Journal of Sichuan Forestry Science and Technology > 2014 > 35(4): 9-12

ZHANG Wei, CHEN Zhong, LONG Han-li, ZHUANG Guo-qing, LI Xiao-qing. Comparison between Albescent and Normal Seedings of Phoebe zhennan by AFLP[J]. Journal of Sichuan Forestry Science and Technology, 2014, 35(4): 9-12. doi: 10.16779/j.cnki.1003-5508.2014.04.002

Citation:

ZHANG Wei, CHEN Zhong, LONG Han-li, ZHUANG Guo-qing, LI Xiao-qing. Comparison between Albescent and Normal Seedings of Phoebe zhennan by AFLP[J]. Journal of Sichuan Forestry Science and Technology, 2014, 35(4): 9-12. doi: 10.16779/j.cnki.1003-5508.2014.04.002

Comparison between Albescent and Normal Seedings of Phoebe zhennan by AFLP

doi: 10.16779/j.cnki.1003-5508.2014.04.002

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Sichuan Academy of Forestry, Chengdu 610081, China;Neijiang Forestry Bureau, Neijiang 641100, China

Received Date: 2014-04-09

Abstract

Using the amplified fragment length polymorphism (AFLP) technique to study polymorphism between the genomic DNA of albescent seedlings and normal seedlings of Phoebe zhennan, it was found that bands amplified by P6 and P11 were between albescent seedlings and normal seedlings. The number of bands of albescent seedlings amplified by P6 was one less than that of normal seedlings and the size of the lacking stripe was about 230 bp. The number of bands of albescent seedlings amplified by P11 was one more than that of normal seedlings and the size of the extra stripe was around 200 bp. The differences of bands indicated that the DNA sequence of albescent seedlings was different from that of normal seedlings. It was needed to conduct sequence and comparative analysis to confirm whether the differences were caused by base mutation,deletion or insertion.
- Albescent seedlings of Phoebe zhennan;
- DNA polymorphism
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Comparison between Albescent and Normal Seedings of Phoebe zhennan by AFLP

doi: 10.16779/j.cnki.1003-5508.2014.04.002

Sichuan Academy of Forestry, Chengdu 610081, China;Neijiang Forestry Bureau, Neijiang 641100, China

Received Date: 2014-04-09

Key words:

Albescent seedlings of Phoebe zhennan /
DNA polymorphism

Abstract: Using the amplified fragment length polymorphism (AFLP) technique to study polymorphism between the genomic DNA of albescent seedlings and normal seedlings of Phoebe zhennan, it was found that bands amplified by P6 and P11 were between albescent seedlings and normal seedlings. The number of bands of albescent seedlings amplified by P6 was one less than that of normal seedlings and the size of the lacking stripe was about 230 bp. The number of bands of albescent seedlings amplified by P11 was one more than that of normal seedlings and the size of the extra stripe was around 200 bp. The differences of bands indicated that the DNA sequence of albescent seedlings was different from that of normal seedlings. It was needed to conduct sequence and comparative analysis to confirm whether the differences were caused by base mutation,deletion or insertion.