The Research Progress on the Preparation and Detection of Ginkgolides in <i>Ginkgo biloba</i> Leaves

YANG Shi-long; MA Xiao-fang

doi:10.16779/j.cnki.1003-5508.2018.06.005

Volume 39 Issue 6

Nov. 2019

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Article Navigation > Journal of Sichuan Forestry Science and Technology > 2018 > 39(6): 25-30

YANG Shi-long, MA Xiao-fang. The Research Progress on the Preparation and Detection of Ginkgolides in Ginkgo biloba Leaves[J]. Journal of Sichuan Forestry Science and Technology, 2018, 39(6): 25-30. doi: 10.16779/j.cnki.1003-5508.2018.06.005

Citation:

YANG Shi-long, MA Xiao-fang. The Research Progress on the Preparation and Detection of Ginkgolides in Ginkgo biloba Leaves[J]. Journal of Sichuan Forestry Science and Technology, 2018, 39(6): 25-30. doi: 10.16779/j.cnki.1003-5508.2018.06.005

The Research Progress on the Preparation and Detection of Ginkgolides in Ginkgo biloba Leaves

doi: 10.16779/j.cnki.1003-5508.2018.06.005

More Information

Advanced Analysis and Testing Center, Nanjing Forestry University, Nanjing 210037, China

Received Date: 2018-08-04

Abstract

Ginkgolides are the main and special ingredients of extracts of Ginkgo biloba leaves. And ginkgolides have many kinds of biological activities. There are little research on ginkgolides because of a smaller amount of ginkgolides in Ginkgo biloba leaves. In this paper,a comprehensive descriptior is made of the extraction, separation, purification and detection methods of ginkgolides. The high purity of ginkgolides could be obtained by choosing suitable solvents and extraction methods with appropriate separation ways, such as column chromatography and solvent extraction method. While higher purity of ginkgolides, even monomers of ginkgolides were obtained by crystallization process, high-speed countercurrent chromatography, which is important to pharmaceuticals industry.
- Ginkgolides;
- Ginkgo biloba leaves;
- Extraction;
- Separation;
- Detection

References

[1]	李文东,高乾善,杨鹏,等.银杏叶中银杏内酯提取工艺研究[J].山东科学,2016,29(5):29~35.
[2]	Grypioti A D,Kostopanagiotou G,Demopoulos C A,et al.Platelet activating factor (PAF) antagonism with ginkgolide B protects the liver against acute injury.importance of controlling the receptor of PAF[J].Digestive Diseases and Sciences.2008,53(4):1054~1062.
[3]	Ma S W,Liu X Y,Xun Q R,et al.Neuroprotective effect of ginkgolide K against H₂O₂-induced PC12 cell cytotoxicity by ameliorating mitochondrial dysfunction and oxidative stress[J].Biological & Pharmaceutical Bulletin.2014,37(2):217~225.
[4]	Hao Y L,Sun Y W,Xu Y,et al.Improvement of contractile function in isolated cardiomyocytes from ischemia-reperfusion rats by ginkgolide B pretreatment[J].Journal of Cardiovascular Pharmacology.2009,54(1):3~9.
[5]	Liu X Y,Zhao G X,Yan Y,et al.Ginkgolide B reduces atherogenesis and vascular inflammation in ApoE^-/- Mice[J].Plos One.2012,7(5):1~10.
[6]	Chan W H.The signaling cascades of ginkgolide B-induced apoptosis in MCF-7 breast cancer cells[J].International Journal of Molecular Sciences.2007,8(11):1177~1195.
[7]	Farooq A,Whitehead D,Azzawi M.Mitochondria protection with ginkgolide B-loaded polymeric nanocapsules prevents diethylnitrosamine-induced hepatocarcinoma in rats[J].Naomedicine,2014,9(3):413~425.
[8]	冷平生,王天华,吴京科,等.银杏萜类内酯的提取和气相色谱与质谱分析[J].北京林业大学学报,2000, (5):19~22.
[9]	戴余军,仇小艳,田春元,等.超声波辅助酶法提取银杏叶总内酯的工艺研究[J].食品科技,2014, 39(12):244~248.
[10]	朱兴一,谢捷,忙怡丽,等.微波辅助提取银杏叶萜类内酯的工艺研究[J].高校化学工程学报,2009,23(6):1080~1083.
[11]	吴昊,宗志敏,李秀秀.超声-微波协同萃取银杏叶黄酮与内酯B的工艺研究[J].中国酿造,2016,35(10):153~156.
[12]
[13]	潘炘,谷西荣,庄晓伟,等.银杏叶超临界CO₂萃取银杏内酯动态变化的研究[J].生物质化学工程,2010,44(3):28~31.
[14]	李文东,郑振佳,高乾善,等.银杏内酯的亚临界水提取工艺研究[J].化学与生物工程,2017,34(5):33~36.
[15]
[16]	王平,陈丹龙,朱兴一,等.闪式提取银杏叶中萜类内酯的工艺研究[J].时珍国医国药,2009,20(11):2825~2826.
[17]	严铸云,罗静,郭晓恒,等.产银杏内酯内生真菌的筛选及培养条件研究[J].天然产物研究与开发,2007(4):554~558.
[18]	包飞,樊明涛,贺江.产银杏内酯B内生真菌的分离与筛选[J].西北农业学报,2008(3):328~331.
[19]	Cui Y N,Yi D W,Bai X F,et al.Ginkgolide B produced endophytic fungus (Fusarium oxysporum) isolated from Ginkgo biloba[J].Fitoterapia,2012,83(5):913~920.
[20]	陈小明,何福林,李志忠,等.产银杏内酯内生真菌的分离与鉴定[J].食品与机械,2017,33(08):27~30.
[21]	王永刚,李守信,向兰.闪式硅胶柱层析法分离银杏黄酮和萜类内酯的工艺研究[J].齐鲁药事,2010,29(7):425~427.
[22]	韩金玉,李海静,李岩,等.大孔吸附树脂对银杏内酯和白果内酯吸附性能的研究[J].离子交换与吸附,2000,16(5):426~431.
[23]	李新岗,黄蕴慧,顾银娜,等.银杏内酯的柱色谱制备[J].中国医药工业杂志,2001(1):4+8.
[24]	王成章,郁青,谭卫红,等.溶剂萃取银杏萜内酯的因子研究[J].天然产物研究与开发,1999(2):53~57.
[25]	陈凡,李奕雅.结晶法纯化银杏内酯的研究[J].福建轻纺,2008(12):43~46.
[26]	郭文生,周波,徐赫男,等.银杏内酯C分子烙印聚合物的制备[J].天然产物研究与开发,2008(2):317~319+331.
[27]	郁青,沈兆邦,袁跃平,等.高含量银杏萜内酯富集方法研究[J].林产化学与工业,2000(2):1~4.
[28]	楼凤昌,凌娅,唐于平,等.银杏萜内酯的分离、纯化和结构鉴定[J].中国天然药物,2004(01):12~16.
[29]	苏静,谈锋,李连强,等.高速逆流色谱法分离纯化银杏叶中白果内酯和银杏内酯A、B、C[J].中草药,2008,39(11):1644~1648.
[30]	秦燕,何新英,庄慧娣,等.HPLC测定银杏提取物中主要黄酮和萜内酯含量的优化方法[J].上海第二医科大学学报,2003(3):234~236.
[31]	张亚中,周亚球,王娟,等.HPLC-ELSD法测定银杏叶软胶囊中萜类内酯的含量[J].中成药,2005(1):26~28.
[32]
[33]	廖咏玲,许锋,朱俊,等.大口径毛细管气相色谱分离和测定银杏叶萜内酯的方法建立[J].西北农业学报,2008(1):146~149.
[34]	Mauri P,Migliazza B,Pietta P.Liquid chromatography/electrospray mass spectrometry of bioactive terpenoids in ginkgo biloba L.[J].Journal of Mass Spectrometry,1999,34:1361~1367.
[35]	陈学国,孔亮,盛亮洪,等.全二维液相色谱串联质谱用于银杏叶提取物成分分析的研究[J].色谱,2005(1):46~51.
[36]	姚评佳,梁锦添,段金友,等.傅里叶红外标准加入法测定银杏叶提取物(EGb)中的总内酯[J].广西农业生物科学,1999(1):51~54.
[37]	张渝阳,王鑫,赵丽娟,等.分光光度法测定银杏萜内酯的含量[J].辽宁化工,2006(10):618~620.
[38]	Choi Y H,Choi H K,Hazekamp A,et al.Quantitative analysis of bilobalide and ginkgolides from ginkgo biloba leaves and ginkgo products using ¹H-NMR[J].Chemical & Pharmaceutical Bulletin,2003,501(2):158~161.
[39]	耿婷,申文雯,王佳佳,等.银杏叶中内酯类成分的研究进展[J].中国中药杂志,2018,43(7):1384~1391.

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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The Research Progress on the Preparation and Detection of Ginkgolides in Ginkgo biloba Leaves

Advanced Analysis and Testing Center, Nanjing Forestry University, Nanjing 210037, China

Received Date: 2018-08-04

Key words:

Abstract: Ginkgolides are the main and special ingredients of extracts of Ginkgo biloba leaves. And ginkgolides have many kinds of biological activities. There are little research on ginkgolides because of a smaller amount of ginkgolides in Ginkgo biloba leaves. In this paper,a comprehensive descriptior is made of the extraction, separation, purification and detection methods of ginkgolides. The high purity of ginkgolides could be obtained by choosing suitable solvents and extraction methods with appropriate separation ways, such as column chromatography and solvent extraction method. While higher purity of ginkgolides, even monomers of ginkgolides were obtained by crystallization process, high-speed countercurrent chromatography, which is important to pharmaceuticals industry.

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Reference (39)

[1]	李文东,高乾善,杨鹏,等.银杏叶中银杏内酯提取工艺研究[J].山东科学,2016,29(5):29~35.
[2]	Grypioti A D,Kostopanagiotou G,Demopoulos C A,et al.Platelet activating factor (PAF) antagonism with ginkgolide B protects the liver against acute injury.importance of controlling the receptor of PAF[J].Digestive Diseases and Sciences.2008,53(4):1054~1062.
[3]	Ma S W,Liu X Y,Xun Q R,et al.Neuroprotective effect of ginkgolide K against H₂O₂-induced PC12 cell cytotoxicity by ameliorating mitochondrial dysfunction and oxidative stress[J].Biological & Pharmaceutical Bulletin.2014,37(2):217~225.
[4]	Hao Y L,Sun Y W,Xu Y,et al.Improvement of contractile function in isolated cardiomyocytes from ischemia-reperfusion rats by ginkgolide B pretreatment[J].Journal of Cardiovascular Pharmacology.2009,54(1):3~9.
[5]	Liu X Y,Zhao G X,Yan Y,et al.Ginkgolide B reduces atherogenesis and vascular inflammation in ApoE^-/- Mice[J].Plos One.2012,7(5):1~10.
[6]	Chan W H.The signaling cascades of ginkgolide B-induced apoptosis in MCF-7 breast cancer cells[J].International Journal of Molecular Sciences.2007,8(11):1177~1195.
[7]	Farooq A,Whitehead D,Azzawi M.Mitochondria protection with ginkgolide B-loaded polymeric nanocapsules prevents diethylnitrosamine-induced hepatocarcinoma in rats[J].Naomedicine,2014,9(3):413~425.
[8]	冷平生,王天华,吴京科,等.银杏萜类内酯的提取和气相色谱与质谱分析[J].北京林业大学学报,2000, (5):19~22.
[9]	戴余军,仇小艳,田春元,等.超声波辅助酶法提取银杏叶总内酯的工艺研究[J].食品科技,2014, 39(12):244~248.
[10]	朱兴一,谢捷,忙怡丽,等.微波辅助提取银杏叶萜类内酯的工艺研究[J].高校化学工程学报,2009,23(6):1080~1083.
[11]	吴昊,宗志敏,李秀秀.超声-微波协同萃取银杏叶黄酮与内酯B的工艺研究[J].中国酿造,2016,35(10):153~156.
[12]
[13]	潘炘,谷西荣,庄晓伟,等.银杏叶超临界CO₂萃取银杏内酯动态变化的研究[J].生物质化学工程,2010,44(3):28~31.
[14]	李文东,郑振佳,高乾善,等.银杏内酯的亚临界水提取工艺研究[J].化学与生物工程,2017,34(5):33~36.
[15]
[16]	王平,陈丹龙,朱兴一,等.闪式提取银杏叶中萜类内酯的工艺研究[J].时珍国医国药,2009,20(11):2825~2826.
[17]	严铸云,罗静,郭晓恒,等.产银杏内酯内生真菌的筛选及培养条件研究[J].天然产物研究与开发,2007(4):554~558.
[18]	包飞,樊明涛,贺江.产银杏内酯B内生真菌的分离与筛选[J].西北农业学报,2008(3):328~331.
[19]	Cui Y N,Yi D W,Bai X F,et al.Ginkgolide B produced endophytic fungus (Fusarium oxysporum) isolated from Ginkgo biloba[J].Fitoterapia,2012,83(5):913~920.
[20]	陈小明,何福林,李志忠,等.产银杏内酯内生真菌的分离与鉴定[J].食品与机械,2017,33(08):27~30.
[21]	王永刚,李守信,向兰.闪式硅胶柱层析法分离银杏黄酮和萜类内酯的工艺研究[J].齐鲁药事,2010,29(7):425~427.
[22]	韩金玉,李海静,李岩,等.大孔吸附树脂对银杏内酯和白果内酯吸附性能的研究[J].离子交换与吸附,2000,16(5):426~431.
[23]	李新岗,黄蕴慧,顾银娜,等.银杏内酯的柱色谱制备[J].中国医药工业杂志,2001(1):4+8.
[24]	王成章,郁青,谭卫红,等.溶剂萃取银杏萜内酯的因子研究[J].天然产物研究与开发,1999(2):53~57.
[25]	陈凡,李奕雅.结晶法纯化银杏内酯的研究[J].福建轻纺,2008(12):43~46.
[26]	郭文生,周波,徐赫男,等.银杏内酯C分子烙印聚合物的制备[J].天然产物研究与开发,2008(2):317~319+331.
[27]	郁青,沈兆邦,袁跃平,等.高含量银杏萜内酯富集方法研究[J].林产化学与工业,2000(2):1~4.
[28]	楼凤昌,凌娅,唐于平,等.银杏萜内酯的分离、纯化和结构鉴定[J].中国天然药物,2004(01):12~16.
[29]	苏静,谈锋,李连强,等.高速逆流色谱法分离纯化银杏叶中白果内酯和银杏内酯A、B、C[J].中草药,2008,39(11):1644~1648.
[30]	秦燕,何新英,庄慧娣,等.HPLC测定银杏提取物中主要黄酮和萜内酯含量的优化方法[J].上海第二医科大学学报,2003(3):234~236.
[31]	张亚中,周亚球,王娟,等.HPLC-ELSD法测定银杏叶软胶囊中萜类内酯的含量[J].中成药,2005(1):26~28.
[32]
[33]	廖咏玲,许锋,朱俊,等.大口径毛细管气相色谱分离和测定银杏叶萜内酯的方法建立[J].西北农业学报,2008(1):146~149.
[34]	Mauri P,Migliazza B,Pietta P.Liquid chromatography/electrospray mass spectrometry of bioactive terpenoids in ginkgo biloba L.[J].Journal of Mass Spectrometry,1999,34:1361~1367.
[35]	陈学国,孔亮,盛亮洪,等.全二维液相色谱串联质谱用于银杏叶提取物成分分析的研究[J].色谱,2005(1):46~51.
[36]	姚评佳,梁锦添,段金友,等.傅里叶红外标准加入法测定银杏叶提取物(EGb)中的总内酯[J].广西农业生物科学,1999(1):51~54.
[37]	张渝阳,王鑫,赵丽娟,等.分光光度法测定银杏萜内酯的含量[J].辽宁化工,2006(10):618~620.
[38]	Choi Y H,Choi H K,Hazekamp A,et al.Quantitative analysis of bilobalide and ginkgolides from ginkgo biloba leaves and ginkgo products using ¹H-NMR[J].Chemical & Pharmaceutical Bulletin,2003,501(2):158~161.
[39]	耿婷,申文雯,王佳佳,等.银杏叶中内酯类成分的研究进展[J].中国中药杂志,2018,43(7):1384~1391.

The Research Progress on the Preparation and Detection of Ginkgolides in Ginkgo biloba Leaves

doi: 10.16779/j.cnki.1003-5508.2018.06.005

Abstract

References

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通讯作者: 陈斌, bchen63@163.com

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The Research Progress on the Preparation and Detection of Ginkgolides in Ginkgo biloba Leaves

doi: 10.16779/j.cnki.1003-5508.2018.06.005

Advanced Analysis and Testing Center, Nanjing Forestry University, Nanjing 210037, China

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The Research Progress on the Preparation and Detection of Ginkgolides in Ginkgo biloba Leaves

doi: 10.16779/j.cnki.1003-5508.2018.06.005

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References

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通讯作者: 陈斌, bchen63@163.com

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Proportional views

The Research Progress on the Preparation and Detection of Ginkgolides in Ginkgo biloba Leaves

doi: 10.16779/j.cnki.1003-5508.2018.06.005

Advanced Analysis and Testing Center, Nanjing Forestry University, Nanjing 210037, China

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