Research on the Aseptic Seeding Technique of <i>Bletilla striata</i> with Lilac Flowers

JIANG Li-qiong; XIAO Xin-yi; LI Wen-jun; XIAO Qian-gang; XU Zhi-ping; LIAO Xing-yong; CHEN Wen-ling; LI Lu

doi:10.16779/j.cnki.1003-5508.2017.06.013

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Volume 38 Issue 6

Nov. 2019

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Article Navigation > Journal of Sichuan Forestry Science and Technology > 2017 > 34(6): 51-54

JIANG Li-qiong, XIAO Xin-yi, LI Wen-jun, XIAO Qian-gang, XU Zhi-ping, LIAO Xing-yong, CHEN Wen-ling, LI Lu. Research on the Aseptic Seeding Technique of Bletilla striata with Lilac Flowers[J]. Journal of Sichuan Forestry Science and Technology, 2017, 34(6): 51-54. doi: 10.16779/j.cnki.1003-5508.2017.06.013

Citation:

JIANG Li-qiong, XIAO Xin-yi, LI Wen-jun, XIAO Qian-gang, XU Zhi-ping, LIAO Xing-yong, CHEN Wen-ling, LI Lu. Research on the Aseptic Seeding Technique of Bletilla striata with Lilac Flowers[J]. Journal of Sichuan Forestry Science and Technology, 2017, 34(6): 51-54. doi: 10.16779/j.cnki.1003-5508.2017.06.013

Research on the Aseptic Seeding Technique of Bletilla striata with Lilac Flowers

doi: 10.16779/j.cnki.1003-5508.2017.06.013

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Chengdu Academy of Agriculture and Forestry Science, Chengdu 611130, China;Chegndu Experimental Foreign Languages School Class, Chengdu 610031, China

Received Date: 2017-07-17

Abstract

Taking the ripe seeds that removed from the inflated pods as explant,experiments were conducted on this tissue culture.The results showed that the best inductive medium for explant culture was an improved fromula listed as follows:MS+0.2 mg·L^-16-BA+0.2 mg·L^-1NAA,and the germination rate could be generally up to 90%~100%;The best enriched medium was an improved formula listed as follows:MS+2.0 mg·L^-16-BA+0.3 mg·L^-1NAA,and the propagation coefficient was 3.4;The best rooting medium was an improved formula of banana puree substrate listed as follows:MS+0.2 mg·L^-1NAA+1 mg·L^-1AC+50 g·L^-1,and the rate of rooting was 95.6%.The growth substrate was a combination of humus:perlite:coconut hust:fluvial sand=5:1:2:2,which was the best transplanting substrate for Bletilla striata,and the survival rate of the transplanting reached 97%.
- Bletilla striata;
- Aseptic seeding
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Research on the Aseptic Seeding Technique of Bletilla striata with Lilac Flowers

doi: 10.16779/j.cnki.1003-5508.2017.06.013

Chengdu Academy of Agriculture and Forestry Science, Chengdu 611130, China;Chegndu Experimental Foreign Languages School Class, Chengdu 610031, China

Received Date: 2017-07-17

Key words:

Bletilla striata /
Aseptic seeding

Abstract: Taking the ripe seeds that removed from the inflated pods as explant,experiments were conducted on this tissue culture.The results showed that the best inductive medium for explant culture was an improved fromula listed as follows:MS+0.2 mg·L^-16-BA+0.2 mg·L^-1NAA,and the germination rate could be generally up to 90%~100%;The best enriched medium was an improved formula listed as follows:MS+2.0 mg·L^-16-BA+0.3 mg·L^-1NAA,and the propagation coefficient was 3.4;The best rooting medium was an improved formula of banana puree substrate listed as follows:MS+0.2 mg·L^-1NAA+1 mg·L^-1AC+50 g·L^-1,and the rate of rooting was 95.6%.The growth substrate was a combination of humus:perlite:coconut hust:fluvial sand=5:1:2:2,which was the best transplanting substrate for Bletilla striata,and the survival rate of the transplanting reached 97%.