The Callus Induction Medium Optimization of Larch

FANG Jiao-yang; FENG De-ming; HE Meng-ying; HE Shu-chun; WANG Jing-xin; ZHANG Lei

doi:10.16779/j.cnki.1003-5508.2015.05.013

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Volume 36 Issue 5

Nov. 2019

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Article Navigation > Journal of Sichuan Forestry Science and Technology > 2015 > 36(5): 72-74,55

FANG Jiao-yang, FENG De-ming, HE Meng-ying, HE Shu-chun, WANG Jing-xin, ZHANG Lei. The Callus Induction Medium Optimization of Larch[J]. Journal of Sichuan Forestry Science and Technology, 2015, 36(5): 72-74,55. doi: 10.16779/j.cnki.1003-5508.2015.05.013

Citation:

FANG Jiao-yang, FENG De-ming, HE Meng-ying, HE Shu-chun, WANG Jing-xin, ZHANG Lei. The Callus Induction Medium Optimization of Larch[J]. Journal of Sichuan Forestry Science and Technology, 2015, 36(5): 72-74,55. doi: 10.16779/j.cnki.1003-5508.2015.05.013

The Callus Induction Medium Optimization of Larch

doi: 10.16779/j.cnki.1003-5508.2015.05.013

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Northeast Forestry University, School of Forestry, Harbin 150040, China

Received Date: 2015-05-27

Abstract

The Larch mature zygotic embryos were taken as the explants. They were disinfected according to different treatment methods,and induced by use of different medium and hormone types and concentration,then the callus volume were calculated. Statistical analysis results showed that the peeled seeds were washed with water for 24 hours~48 hours,disinfected in 70% alcohol for 1 minute and disinfected into 4% NaClO for 10 minutes,and then the embryo was stripped out in the 0.5% NaClO disinfection for 2 minutes and the suvival rate of embryos was the highest; In the light conditions,MS medium was good medium for callus induction. It could be seen that 2.0 mg·L^-1 2,4-D + 1.0 mg·L^-1 6-BA or 1.0 mg·L^-1 NAA + 0.5 mg·L^-1 6-BA were the best hormone combination.
- Larch;
- Callus;
- Inducation
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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The Callus Induction Medium Optimization of Larch

doi: 10.16779/j.cnki.1003-5508.2015.05.013

Northeast Forestry University, School of Forestry, Harbin 150040, China

Received Date: 2015-05-27

Key words:

Larch /
Callus /
Inducation

Abstract: The Larch mature zygotic embryos were taken as the explants. They were disinfected according to different treatment methods,and induced by use of different medium and hormone types and concentration,then the callus volume were calculated. Statistical analysis results showed that the peeled seeds were washed with water for 24 hours~48 hours,disinfected in 70% alcohol for 1 minute and disinfected into 4% NaClO for 10 minutes,and then the embryo was stripped out in the 0.5% NaClO disinfection for 2 minutes and the suvival rate of embryos was the highest; In the light conditions,MS medium was good medium for callus induction. It could be seen that 2.0 mg·L^-1 2,4-D + 1.0 mg·L^-1 6-BA or 1.0 mg·L^-1 NAA + 0.5 mg·L^-1 6-BA were the best hormone combination.