An Experimental Study of Tissue Culture of <i>Chamelaucium uncinatum</i> ‘Snow Flake’

LOU Li-hua; ZENG Jing; JIANG Hai-yan; YAN Qiao; WANG Zheng-chun

doi:10.16779/j.cnki.1003-5508.2014.02.008

WE ARE COMMITTED TO REPORTING THE LATEST FORESTRY ACADEMIC ACHIEVEMENTS

PDF
Cite
Share

facebook

twitter

google

linkedin

Volume 35 Issue 2

Nov. 2019

Article Contents

Turn off MathJax

Article Navigation > Journal of Sichuan Forestry Science and Technology > 2014 > 35(2): 34-36,56

LOU Li-hua, ZENG Jing, JIANG Hai-yan, YAN Qiao, WANG Zheng-chun. An Experimental Study of Tissue Culture of Chamelaucium uncinatum ‘Snow Flake’[J]. Journal of Sichuan Forestry Science and Technology, 2014, 35(2): 34-36,56. doi: 10.16779/j.cnki.1003-5508.2014.02.008

Citation:

LOU Li-hua, ZENG Jing, JIANG Hai-yan, YAN Qiao, WANG Zheng-chun. An Experimental Study of Tissue Culture of Chamelaucium uncinatum ‘Snow Flake’[J]. Journal of Sichuan Forestry Science and Technology, 2014, 35(2): 34-36,56. doi: 10.16779/j.cnki.1003-5508.2014.02.008

An Experimental Study of Tissue Culture of Chamelaucium uncinatum ‘Snow Flake’

doi: 10.16779/j.cnki.1003-5508.2014.02.008

More Information

Chongqing Academy of Forestry, Chongqing Key Laboratory of Three Gorges Area Forest Ecology Protection and Restoration, Chongqing 400036, China

Received Date: 2013-12-04

Abstract

As a new generation of woody cut flowers, C. uncinatum ‘Snow Flake’ has a high ornamental and economic value. Based on the study of tissue culture of ‘Snow Flake’,the aim of this experiment was to lay the foundation for Industrial production of ‘Snow Flake’. The results showed that 7 minutes were the best time to sterilize explants by using 0.1% HgCl2. MS1 + 6-BA1.0 mg·L^-1 + IBA0.2 mg·L^-1 + KT0.5 mg·L^-1 was the optimum primary culture medium, the best subcultuer medium was WPM1 + 6-BA 1.0 mg·L^-1 + IBA 0.2 mg·L^-1 + GA3 1.5 mg·L^-1,multiplication coefficient was 4.2,multiplication was 3~6. The most suitable rooting medium was 1/2MS2 + IBA0.2 mg·L^-1 + 0.1 mg·L^-1 GGR.
- Chamelaucium uncinatum;
- Tissue culture;
- Proliferation;
- Rooting
References
Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article views(257) PDF downloads(4) Cited by()

Proportional views

An Experimental Study of Tissue Culture of Chamelaucium uncinatum ‘Snow Flake’

doi: 10.16779/j.cnki.1003-5508.2014.02.008

Chongqing Academy of Forestry, Chongqing Key Laboratory of Three Gorges Area Forest Ecology Protection and Restoration, Chongqing 400036, China

Received Date: 2013-12-04

Key words:

Abstract: As a new generation of woody cut flowers, C. uncinatum ‘Snow Flake’ has a high ornamental and economic value. Based on the study of tissue culture of ‘Snow Flake’,the aim of this experiment was to lay the foundation for Industrial production of ‘Snow Flake’. The results showed that 7 minutes were the best time to sterilize explants by using 0.1% HgCl2. MS1 + 6-BA1.0 mg·L^-1 + IBA0.2 mg·L^-1 + KT0.5 mg·L^-1 was the optimum primary culture medium, the best subcultuer medium was WPM1 + 6-BA 1.0 mg·L^-1 + IBA 0.2 mg·L^-1 + GA3 1.5 mg·L^-1,multiplication coefficient was 4.2,multiplication was 3~6. The most suitable rooting medium was 1/2MS2 + IBA0.2 mg·L^-1 + 0.1 mg·L^-1 GGR.