The Optimization of the SSR Reaction Program and the Reaction System in Researches on <i>Quercus liaotungensis</i>

KANG Feng-feng; QIN Ying-ying; HAN Hai-rong

doi:10.16779/j.cnki.1003-5508.2013.04.001

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Volume 34 Issue 4

Nov. 2019

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Article Navigation > Journal of Sichuan Forestry Science and Technology > 2013 > 34(4): 1-4

KANG Feng-feng, QIN Ying-ying, HAN Hai-rong. The Optimization of the SSR Reaction Program and the Reaction System in Researches on Quercus liaotungensis[J]. Journal of Sichuan Forestry Science and Technology, 2013, 34(4): 1-4. doi: 10.16779/j.cnki.1003-5508.2013.04.001

Citation:

KANG Feng-feng, QIN Ying-ying, HAN Hai-rong. The Optimization of the SSR Reaction Program and the Reaction System in Researches on Quercus liaotungensis[J]. Journal of Sichuan Forestry Science and Technology, 2013, 34(4): 1-4. doi: 10.16779/j.cnki.1003-5508.2013.04.001

The Optimization of the SSR Reaction Program and the Reaction System in Researches on Quercus liaotungensis

doi: 10.16779/j.cnki.1003-5508.2013.04.001

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Key Laboratory for Silviculture and Forest Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, China

Received Date: 2013-04-24

Abstract

In this paper,optimization researches were conducted on the SSR reaction program and the reaction system for the research process of Q. liaotungensis genetic diversity. The optimized reaction program was to adopt 33 cycles,which included 3 minutes of pre-denaturing at 94℃,45 seconds of denaturing at 94℃,45 seconds of annealing at 58℃,45 seconds of extension at 72℃,and a final extension step was carried out at 72℃ for 7 minutes. The optimized reaction system was as follows:30 ng DNA,2 μL10×Buffer(Mg²⁺ Plus),150 μmol·L^-1 dNTP Mixture,0.3 μmol·L^-1of each primer,1U(5 u·μL^-1) Taq polymerase,and the total reaction volume was 20 μL. The results had a certain reference value for the application of the SSR marker in the research on the genetic diversity of oak plants.
- Quercus liaotungensis;
- SSR;
- Reaction program;
- The reaction system;
- Optimization
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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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The Optimization of the SSR Reaction Program and the Reaction System in Researches on Quercus liaotungensis

doi: 10.16779/j.cnki.1003-5508.2013.04.001

Key Laboratory for Silviculture and Forest Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, China

Received Date: 2013-04-24

Key words:

Abstract: In this paper,optimization researches were conducted on the SSR reaction program and the reaction system for the research process of Q. liaotungensis genetic diversity. The optimized reaction program was to adopt 33 cycles,which included 3 minutes of pre-denaturing at 94℃,45 seconds of denaturing at 94℃,45 seconds of annealing at 58℃,45 seconds of extension at 72℃,and a final extension step was carried out at 72℃ for 7 minutes. The optimized reaction system was as follows:30 ng DNA,2 μL10×Buffer(Mg²⁺ Plus),150 μmol·L^-1 dNTP Mixture,0.3 μmol·L^-1of each primer,1U(5 u·μL^-1) Taq polymerase,and the total reaction volume was 20 μL. The results had a certain reference value for the application of the SSR marker in the research on the genetic diversity of oak plants.