北川驴蹄草(<i>Caltha dysosmoides</i>)花部特征研究

别鹏飞; 谢欢欢; 胡进耀; 朱云东

doi:10.16779/j.cnki.1003-5508.2017.02.003

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北川驴蹄草(Caltha dysosmoides)花部特征研究

别鹏飞 , 谢欢欢 , 胡进耀 , 朱云东

文章导航 > 四川林业科技 > 2017 > 38(2): 13-16

别鹏飞, 谢欢欢, 胡进耀, 朱云东. 北川驴蹄草(Caltha dysosmoides)花部特征研究[J]. 四川林业科技, 2017, 38(2): 13-16. doi: 10.16779/j.cnki.1003-5508.2017.02.003

引用本文:

别鹏飞, 谢欢欢, 胡进耀, 朱云东. 北川驴蹄草(Caltha dysosmoides)花部特征研究[J]. 四川林业科技, 2017, 38(2): 13-16. doi: 10.16779/j.cnki.1003-5508.2017.02.003

BIE Peng-fei, XIE Huan-huan, HU Jin-yao, ZHU Yun-dong. Pollen Viability,Stigma Receptivity and Floral Characteristics Study of Caltha dysosmoides[J]. Journal of Sichuan Forestry Science and Technology, 2017, 38(2): 13-16. doi: 10.16779/j.cnki.1003-5508.2017.02.003

Citation:

BIE Peng-fei, XIE Huan-huan, HU Jin-yao, ZHU Yun-dong. Pollen Viability,Stigma Receptivity and Floral Characteristics Study of Caltha dysosmoides[J]. Journal of Sichuan Forestry Science and Technology, 2017, 38(2): 13-16. doi: 10.16779/j.cnki.1003-5508.2017.02.003

北川驴蹄草(Caltha dysosmoides)花部特征研究

doi: 10.16779/j.cnki.1003-5508.2017.02.003

详细信息

绵阳师范学院，四川绵阳 621000;绵阳蜀创农业科技有限公司，四川绵阳 621000;北川林业局，四川北川 622750

作者简介:
别鹏飞(1990-),男,硕士研究生,绵阳师范学院环境工程专业

基金项目:
片口保护区2014年省级财政林业自然保护区建设项目(2015))29号。

国家自然科学基金项目(31170319)
中图分类号: S722.8

Pollen Viability,Stigma Receptivity and Floral Characteristics Study of Caltha dysosmoides

More Information

Mianyang Normal University, Mianyang 621000;Mianyang Shu Chuang Agricultural Science and Technology Co. Ltd, Mianyang 621000;Beichuan Forestry Bureau, Beichuan 622750, China

摘要: 北川驴蹄草(Caltha dysosmoides Tao Zhang et al)是2016年新命名的物种(四川特有),为保护利用这一新种,急需研究其生殖生物学特征。本研究于不同种群中选取50株进行标记,观察其花部特征,测试花粉活力以及柱头可授性。采用TTC法测定花粉活力;采用液体培养基对北川驴蹄草花粉进行离体培养;采用联苯胺-过氧化氢法测定柱头可授性(直到无活力为止)。实验结果表明:在自然条件下,北川驴蹄草4月~5月开花,单花花期一般为2 d。雄蕊短于雌蕊,花药紧贴花柱外侧,花药与柱头相距约3 mm,在整个花朵开放的过程中雌蕊和雄蕊的相对位置不变;花粉活力早上明显低于下午,且于花后1 h~4 h内增长较快,4h之后花粉活力增长较慢;柱头具有可授性,于花后3 h时柱头可授性相对较强。
- 北川驴蹄草;
- 花部特征;
- 花粉活性;
- 花粉萌发;
- 柱头可授性
Abstract: Caltha dysosmoides is a newly named species in 2016 (specific to Sichuan).For the protection and use of this new species,the urgent need is to study their reproductive biology.In this paper,50 strains were selected from different populations to observe their floral characteristics,pollen viability and stigma receptivity.The pollen viability was measured by TTC method,and the pollen culture of Caltha was cultured in liquid medium,and the stigma receptivity was determined by benzidine hydrogen peroxide (until it was inactive).The results of the investigation showed that under natural conditions,the Caltha bloomed from April to May,and the flowering period of one flower is generally 2 days.Stamens were shorter than pistil; Anthers were close to the outer side of the stilus with a distance of about 3 mm; In the whole process of flower opening,the relative position of the pistil and stamen were invariant; In the morning,pollen viability was significantly lower than in the afternoon,and increased rapidly in 1~4 hours after anthesis,after 4 hours,the pollen activity increased slowly.The stigma could be granted,and it would be relatively strong at 3 hours after anthesis.
- Caltha dysosmoides Tao Zhang et al;
- Floral characteristics;
- Pollen viability;
- Pollen germination;
- Stigma receptivity

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北川驴蹄草(Caltha dysosmoides)花部特征研究

doi: 10.16779/j.cnki.1003-5508.2017.02.003

绵阳师范学院，四川绵阳 621000;绵阳蜀创农业科技有限公司，四川绵阳 621000;北川林业局，四川北川 622750

作者简介:
别鹏飞(1990-),男,硕士研究生,绵阳师范学院环境工程专业

收稿日期: 2017-01-03

基金项目:

片口保护区2014年省级财政林业自然保护区建设项目(2015))29号。

国家自然科学基金项目(31170319)

中图分类号: S722.8

关键词:

摘要: 北川驴蹄草(Caltha dysosmoides Tao Zhang et al)是2016年新命名的物种(四川特有),为保护利用这一新种,急需研究其生殖生物学特征。本研究于不同种群中选取50株进行标记,观察其花部特征,测试花粉活力以及柱头可授性。采用TTC法测定花粉活力;采用液体培养基对北川驴蹄草花粉进行离体培养;采用联苯胺-过氧化氢法测定柱头可授性(直到无活力为止)。实验结果表明:在自然条件下,北川驴蹄草4月~5月开花,单花花期一般为2 d。雄蕊短于雌蕊,花药紧贴花柱外侧,花药与柱头相距约3 mm,在整个花朵开放的过程中雌蕊和雄蕊的相对位置不变;花粉活力早上明显低于下午,且于花后1 h~4 h内增长较快,4h之后花粉活力增长较慢;柱头具有可授性,于花后3 h时柱头可授性相对较强。

English Abstract

Pollen Viability,Stigma Receptivity and Floral Characteristics Study of Caltha dysosmoides

Mianyang Normal University, Mianyang 621000;Mianyang Shu Chuang Agricultural Science and Technology Co. Ltd, Mianyang 621000;Beichuan Forestry Bureau, Beichuan 622750, China

Received Date: 2017-01-03

Keywords:

Abstract: Caltha dysosmoides is a newly named species in 2016 (specific to Sichuan).For the protection and use of this new species,the urgent need is to study their reproductive biology.In this paper,50 strains were selected from different populations to observe their floral characteristics,pollen viability and stigma receptivity.The pollen viability was measured by TTC method,and the pollen culture of Caltha was cultured in liquid medium,and the stigma receptivity was determined by benzidine hydrogen peroxide (until it was inactive).The results of the investigation showed that under natural conditions,the Caltha bloomed from April to May,and the flowering period of one flower is generally 2 days.Stamens were shorter than pistil; Anthers were close to the outer side of the stilus with a distance of about 3 mm; In the whole process of flower opening,the relative position of the pistil and stamen were invariant; In the morning,pollen viability was significantly lower than in the afternoon,and increased rapidly in 1~4 hours after anthesis,after 4 hours,the pollen activity increased slowly.The stigma could be granted,and it would be relatively strong at 3 hours after anthesis.