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SSR即简单序列重复(Simple sequence repeats)又称为微卫星(Microsatellites)[1-2],是以如(CA)n,(TG)n,(GGC)n等少数几个核苷酸为单位的串联重复DNA序列,其中n代表重复次数,重复次数从几个到几十个不等。不同等位基因之间的重复数存在着显著差异,并且重复序列两端多是相对保守的单拷贝序列,可通过设计特异性引物对基因组总DNA进行PCR扩增,扩增片段的长度多态性可用作分子标记[3]。SSR分子标记是群体遗传结构分析、品种遗传多样性研究、品种保护与纯度鉴定、交配系统分析的有效工具,也被认为是信息含量高、多态性高;稳定性好,重复性好;分辨率高、揭示力强的分子标记。
竹类植物是禾本科中最大的自然类群,全世界约有88属1 400种,中国有34属近550种,集材用、笋用和观赏等众多用途,是重要的园林树种之一[4-5]。竹类植物作为一类生物学特性特殊的植物,近年来,虽然SSR分子标记技术在其种质鉴定、种属分类及系统发育等方面有所应用,但是竹类植物中开发的SSR引物较少,检测的SSR位点不足以满足种质鉴定及群体遗传研究的需要,因而需要开发更多的竹类植物SSR引物,来扩大竹类植物种质鉴定的数量和范围及进行系统分类、群体遗传等研究。本文综述了竹类植物中的SSR引物开发方法及研究进展,为进一步研究竹类植物种质资源的群体遗传学提供技术支持。
Exploitation Strategies and Applications of SSR Primer in Bamboo Plants
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摘要: 微卫星(SSR)指的是以核苷酸为单位,串联重复的DNA序列,分布于真核生物的整个基因组中。SSR标记是基于DNA长度多态性的分子标记技术,广泛应用于群体遗传结构分析、构建遗传图谱、基因定位、亲缘关系等领域。本文综述了竹类植物中的SSR引物开发方法及应用,旨在为SSR分子标记在竹类植物中的应用提供参考。Abstract: Microsatellites (SSR) refer to DNA sequences that are repeated in series with nucleotides as units, and widely distributed throughout the genome of eukaryotes. SSR marker is a molecular maker technology based on DNA length polymorphism, which is widely used in the fields of population genetic structure analysis, establishment of genetic map, gene location, genetic relationship, and so on. In this paper, the exploitation methods and applications of SSR primers in bamboo plants were reviewed, aiming to provide useful information for SSR molecular marker in bamboo plants.
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Key words:
- Bamboo plants;
- SSR Primer;
- Exploitation strategy;
- Application
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