Abstract: There were three main hypotheses of spatial coexistence between wild animals and human beings, which were co-existence at regional scale, co-existence at intermediate scale and co-existence at fine scale. Identifying the space utilization mode of humans and wild animals could provide scientific reference for the protection and management of wild animals. Therefore, infrared cameras were used to monitor the activities of giant pandas (Ailuropoda melanoleuca) and human activities in the centralized distribution area of giant pandas in Wolong Nature Reserve. The coexistence mechanism between giant panda and humans was discussed by analyzing the spatial distribution characteristics of giant pandas and human activities. The results showed that (1) at the beginning of the study (2011.11-2013.04), the monthly relative abundance of human activities was relatively low, while the monthly relative abundance of giant pandas detected in this region was relatively high. However, since May 2013, the intensity of human activities increased sharply and stably at a high level, which resulted in a sharp decrease in the monthly relative abundance of giant pandas. (2) Although the use of key limiting factors (such as water sources) led to the overlaps of giant pandas and human activities, in general, the occurrence frequency of giant pandas significantly reduced in regions where human activities frequently occurred. Based on analysis of the three mechanisms of human and wildlife coexistence, the coexistence mode was co-existence at intermediate scale between giant pandas and human activities. Human activities restricted the survival and development of wild animals to a certain extent. The management department should fully consider the impact of human disturbance activities on giant pandas, formulate policies for sustainable development, meanwhile, reduce competition for space and resources between humans and giant pandas.