Abstract: The rooting rate of olive cuttings of Frantoio and E'zhi varieties was studied by immersing them in rooting solution with concentration of 0 mg·L^-1, 100 mg·L^-1, 200 mg·L^-1, 300 mg·L^-1 and 400 mg·L^-1, respectively. The results showed that the cuttings without rooting solution had little callus and hardly rooted.The rooting rate of Frantoio was 3%, 37%, 42%, 76%, 63% in rooting solution of 0 mg·L^-1, 100 mg·L^-1, 200 mg·L^-1, 200 mg·L^-1, 300 mg·L^-1 and 400 mg·L^-1, respectively. The rooting rate of E'zhi was 2%, 21.25%, 40.5%, 70.25% and 53.13% in rooting solution of 0 mg·L^-1, 100 mg·L^-1, 200 mg·L^-1, 300 mg·L^-1 and 400 mg·L^-1,respectively. The optimum concentration of hormones was 300 mg·L^-1 in rooting solution of Olive cuttings.