Abstract: In order to find out the distribution of kiwifruit canker disease in the field,the spatial distribution pattern of the disease was determined by classical aggregation index method and regression analysis method in this study.The results showed that canker-diseased kiwifruit plants showed aggregation distribution in the field,and the basic form of distribution was individual population.The optimum theoretical and sequential sampling numbers were calculated respectively for field sampling of Kiwifruit canker disease,and the optimum sampling square size was determined.The efficiency of investigation sampling was effectively improved through the above research courses,thus providing reference for the prevention of this disense.