长白落叶松愈伤组织及不定芽的诱导

Induction of Callus and Adventitious Bud in Larix olgensis

摘要: 以长白落叶松成熟合子胚为研究对象，从外植体消毒方式及愈伤组织与不定芽诱导的适宜培养基、激素组合和配比的选取等方面着手，进行长白落叶松愈伤组织及不定芽诱导的研究。结果表明：先用4%NaClO消毒去皮种子15 min后，再用0.5%NaClO消毒胚2 min为长白落叶松成熟合子胚的最佳消毒方法；BM为愈伤组织诱导的最适培养基；当BM+2.0 mg·L^-1 TDZ时，愈伤组织诱导率最高，为89%；BM+1.5 mg·L^-1 TDZ时，不定芽诱导率最高，为80.0%。

Abstract: The mature zygotic embryo of Larix olgensis was used as the research object.The callus and adventitious bud induction experiments were made to select disinfection method of explants, appropriate medium and hormone combination for L.olgensis.The optimum disinfection method was to sterilize peeled seeds with 4% NaClO for 15 min,and to disinfect the embryos with 0.5% NaClO for 2 min;BM was the optimum medium for callus induction;In BM medium supplemented with 2.0 mg·l^-1 TDZ,the callus induction reached the highest rate,89%;In BM medium combined with 1.5 mg·l^-1 TDZ,the adventitious bud induction reached the highest rate,80.0%.