利用秋水仙碱诱导美洲黑杨2n花粉的初步研究

A Study of Inducing 2n Pollen of Populus deltoid by Colchicines

摘要: 为了获得美洲黑杨三倍体植株，本试验采用二因素随机区组试验设计，对利用秋水仙碱诱导美洲黑杨2n花粉技术进行研究，结果表明：（1）水培3 d、4 d，5 d时，利用0.3%、0.5%、0.8%的秋水仙碱对美洲黑杨雄花芽进行处理均能得到一定比例的2n花粉；（2）在水培3 d时，利用0.5%的秋水仙碱处理美洲黑杨雄花芽，得到的2n花粉最多，为23.4%。

Abstract: In order to get the triploids of P.deltoid,the technique was researched to induce 2n pollen of P.deltoid by colchicines by two-factor randomized block design.The results indicated that(1)a certain proportion of 2n pollen grains could be gotten from the male buds induced by cochicine solution with the concentration of 0.3%,0.5% and 0.8%,under the condition of water culture for 3,4 and 5 days.(2)the proportion of 2n pollen grains could be the highest when the male buds were treated by the cochicine solution of 0.5% under the condition of water culture for 3 days,the percentage of A4(d≧50) could be up to 1.4%,the percentage of 2n pollen (d≧37.5) could be up to 23.4%.