Abstract: It aimed to gain highly efficient phosphate-solubilizing microorganisms.Phosphate-solubilizing bacteria were isolated in advantage of Pikovskava phosphate dissolving medium.Liquid fermentation experiments showed the phosphate-solubilizing activity,molecular biological methods identified the microbial functional community with higher activity.By single factor test,the vitality of different strains were compared under different formulas and the fermentation conditions of culture medium.Bacillus sp.was the phosphate-solubilizing bacterium with higher efficiency.The optimum medium was as follows:suitable initial pH of 5~9,optimum temperature range of 16℃~40℃,ammonium sulphate,ammonium chloride and ammonium oxalate,peptone for suitable nitrogen source,ammonium oxalate,sucrose,glycerol for suitable carbon source.High concentration of Fe³⁺ and Mn²⁺ had a certain role in promoting its vitality,but Cu²⁺,Ni²⁺ and Zn²⁺ would hold the phosphate solubilizing.In different formulations and fermentation conditions,Its phosphate solubilizing ability was better than that of microbial fertilizer 2.In different fermentation conditions,the stability of phosphate solubilizing ability was better than that of microbial fertilizer 1.It would give a certain market potential for the phosphate-solubilizing bacteria added to the fertilizer.