Abstract: The experiment was carried out with five-leaf and seven-leaf varieties from Pingli county of Shanxi, by comparing the 4 factors of cutting sites (twigs, old branches), number of knots, mulching film and drug concentration. The results showed that the survival rate of the old branches was significantly higher than that of twigs. In the case of cuttage with mulching film (F group), the growth potential was slightly better than that of no-film-covered (CK group);The higher the concentration of α-NAA, the larger the rooting number. Concentration variation of α-NAA showed no significant influence on the growth of Gynostemma pentaphyllum. When the concentration of α-NAA was 300cc, the growth was the best. There were significant differences respectively in vine length, root length and root number of 7 groups.