Abstract: The technology of intersectional crossing between P. deltoides and P. davidiana was studied by five different treatments. And the hybrids and their parents were identified using morphological marker. The results showed that hybrids offspring of P.liaoningensis×P. davidiana were inclined to partial parent type, intermediate type and paternal parent type, while hybrids offspring of P.davidiana×P.liaoningensis were inclined to partial parent type and paternal parent type, which meant the hybrid progeny was true hybrids. The effect was better to break incompatible barriers by fumigating stigma with n-hexane followed by brushing stigma with the fluid extracted from pollen.