Abstract: To study the organogenesis approaches in tissue culture of stem tips of Benihoppe strawberry (Fragaria ananassa Duch.),stem tips were used as explants for tissue culture,aiming to observe the morphological and anatomical changes during organic regeneration.Results showed that the regenerative bud primodium in vitro of Benihoppe Strawberry (F. ananassa Duch.) derived from the parenchyma cells in epidermis on the morphologic apex of stem tips,so it originated in exogenous way;Parenchyma cells in epidermis differentiated to form bud primordium,then the leaf primordium would be differentiated from parenchyma cells in epidermis near it;the bud primordium and leaf primordium grew together to form adventitious shoots;Organogenesis type was the way for the bud primordium to regenerate.The tissue of adventitious shoots base was induced by hormone, which would cause the differentiation of root primordium in cambium, the epidermic cells of adventitious shoots base could form root primordium too.Therefore, the way that adventitious roots originated could be exogenous or endogenous, but the adventitious roots were all the induced root primordia. At last, the conducting tissue in adventitious shoots and roots connected the whole tissue culture seedling into a complete plant.