Abstract: A method was described to make the glass slide specimen for aphids. Reagents included the mixed solution of saturated chloral hydrate and Phenol, 10%NAOH, Ethanol and the others. The treatments of aphid included the structural fixation of aphid, exclusion of impurity, transparency of aphid, dehydration of aphids shaping of aphids which could make the production of aphid glass slide specimens complete and beautiful, being of high success rate and conducive to the identification and long-term preservation of aphid specimens.