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李姝江, 彭娇洋, 朱涵明月, 刘倩, 王诗玮, 朱天辉, 黄祖惠, 吴继云, 张霞. 莱氏野村菌(Nomuraearileyi)固液双相发酵配方研究[J]. 四川林业科技, 2017, 38(5): 33-37. DOI: 10.16779/j.cnki.1003-5508.2017.05.007
引用本文: 李姝江, 彭娇洋, 朱涵明月, 刘倩, 王诗玮, 朱天辉, 黄祖惠, 吴继云, 张霞. 莱氏野村菌(Nomuraearileyi)固液双相发酵配方研究[J]. 四川林业科技, 2017, 38(5): 33-37. DOI: 10.16779/j.cnki.1003-5508.2017.05.007
LI Shu-jiang, PENG Jiao-yang, ZHU Hanmingyue, LIU Qian, WANG Shi-wei, ZHU Tian-hui, HUANG Zu-hui, WU Ji-yun, ZHANG Xia. Optimization of Solid-liquid Biphasic Ferment Constitution of Nomuraea rileyi[J]. Journal of Sichuan Forestry Science and Technology, 2017, 38(5): 33-37. DOI: 10.16779/j.cnki.1003-5508.2017.05.007
Citation: LI Shu-jiang, PENG Jiao-yang, ZHU Hanmingyue, LIU Qian, WANG Shi-wei, ZHU Tian-hui, HUANG Zu-hui, WU Ji-yun, ZHANG Xia. Optimization of Solid-liquid Biphasic Ferment Constitution of Nomuraea rileyi[J]. Journal of Sichuan Forestry Science and Technology, 2017, 38(5): 33-37. DOI: 10.16779/j.cnki.1003-5508.2017.05.007

莱氏野村菌(Nomuraearileyi)固液双相发酵配方研究

Optimization of Solid-liquid Biphasic Ferment Constitution of Nomuraea rileyi

  • 摘要: 莱氏野村菌(Nomuraea rileyi)通过基础培养基基质、碳、氮、维生素筛选,玉米(50 g)、葡萄糖(1.5 g)、水解酪蛋白(1.5 g)、抗坏血酸(0.5 mg)、水50 mL为最佳固体培养基质(培养10 d),产孢量达4.78×1010 cfu·mL-1。以SMY为液体培养基质,莱氏野村菌最适发酵条件:1.0×108 cfu·mL-1接种浓度,25℃、pH=6、全天光照、180 r·min-1振荡培养7 d,菌丝干重为690.2 mg。固体发酵为复合粉炮生产提供基础配方,液体发酵缩短时间后可作为固体培养生产莱氏野村菌菌粉的二级种子,发酵产品为杜仲梦尼夜蛾粉炮防治重要生物制剂。

     

    Abstract: Screening by substrates of basic medium, C, N, and vitamin, the formula of corn(50 g), glucose(1.5 g), casamino acids(1.5 g), ascorbic acid(0.5 mg), H2O(50 mL) was optimal medium substrates(cultivation for 10 d) for Nomuraea rileyi, the sporulation quantity was 4.78×1010 cfu·mL-1. Taking SMY as the liquid medium, the optimal fermentation conditions of N. rileyi were as below:1.0×108 cfu·mL-1 of inoculated concentration, 25℃, pH6, full light, 180 r·min-1 of shaking culturing for 7 days, and 690.2 mg of mycelia. The solid fermentation provided the basic formula for producing compound powder, and shortened time of liquid fermentation could be used as secondary generation for producing N. rileyi powder of solid fermentation. The fermented product is the important biological preparation of control Orthosia songi.

     

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