用微信扫码二维码

分享至好友和朋友圈

WE ARE COMMITTED TO REPORTING THE LATEST FORESTRY ACADEMIC ACHIEVEMENTS

安小燕, 黄燕, 丁利, 干少雄, 毛晓陶, 李书彬. 基于形态特征和线粒体12S、Cyt b和ND4基因片段的横斑锦蛇和玉斑锦蛇的关系研究[J]. 四川林业科技, 2017, 38(2): 85-90. DOI: 10.16779/j.cnki.1003-5508.2017.02.015
引用本文: 安小燕, 黄燕, 丁利, 干少雄, 毛晓陶, 李书彬. 基于形态特征和线粒体12S、Cyt b和ND4基因片段的横斑锦蛇和玉斑锦蛇的关系研究[J]. 四川林业科技, 2017, 38(2): 85-90. DOI: 10.16779/j.cnki.1003-5508.2017.02.015
AN Xiao-yan, HUANG Yan, DING Li, GAN Shao-xiong, MAO Xiao-tao, LI Shu-bin. Relationships Between Elaphe perlacea and Elaphe mandarinus Based on Morphological Characters and Mitochondrial 12S, Cyt b and ND4 Gene Sequences[J]. Journal of Sichuan Forestry Science and Technology, 2017, 38(2): 85-90. DOI: 10.16779/j.cnki.1003-5508.2017.02.015
Citation: AN Xiao-yan, HUANG Yan, DING Li, GAN Shao-xiong, MAO Xiao-tao, LI Shu-bin. Relationships Between Elaphe perlacea and Elaphe mandarinus Based on Morphological Characters and Mitochondrial 12S, Cyt b and ND4 Gene Sequences[J]. Journal of Sichuan Forestry Science and Technology, 2017, 38(2): 85-90. DOI: 10.16779/j.cnki.1003-5508.2017.02.015

基于形态特征和线粒体12S、Cyt b和ND4基因片段的横斑锦蛇和玉斑锦蛇的关系研究

Relationships Between Elaphe perlacea and Elaphe mandarinus Based on Morphological Characters and Mitochondrial 12S, Cyt b and ND4 Gene Sequences

  • 摘要: 本文通过对横斑锦蛇(Elaphe perlacea)肌肉线粒体DNA的提取,对其中的3个基因-12SRNA、ND4和细胞色素b进行扩增、测序,与玉斑锦蛇(Elaphe mandarinus)及锦蛇属(Elaphe)17个物种的相同基因片段重建系统发育树。系统发育结果表明横斑锦蛇与玉斑锦蛇系统发育关系最近,但是两者不是同一个物种。最后将横斑锦蛇和玉斑锦蛇的外部特点进行对比,发现横斑锦蛇与玉斑锦蛇在形态特征上存在稳定性差异。

     

    Abstract: Elaphe perlacea belongs to Elaphe,Megapodiidae.Stejneger collected a male specimen in Ya'an and named it Elaphe perlacea in 1929.The number of Elaphe perlacea is rare,and its distribution areas are limited.Elaphe perlacea is the least studied species of Elaphe.Elaphe mandarinus also belongs to Elaphe,and it was named in 1882.Due to the similarities in heads and tails of both species and the limited information,it is difficult to differentiate the two species.In 2010,a specimen of Elaphe perlacea was collected in Fengtongzhai Nature Reserve of Ya'an.We obtained DNA sequence data of Elaphe perlacea (complete sequences of the mitochondrial NADH dehydrogenase subunit 4ND4 gene,cytochrome b gene and 12S rRNA partial sequences) and established phylogeny trees to examine the relationship of Elaphe species.The phylogenetic tree showed that Elaphe perlacea and Elaphe mandarinus were sister species,but also two valid species.And we also examined the morphological characters of Elaphe perlacea and Elaphe mandarinus,and found that there were stable differences between them.

     

/

返回文章
返回