峨眉金线莲种子无菌苗组培快繁体系的建立

Establishment of Rapid Propagation for sterile Seedlings of Anoectochilus emeiensis by Tissue Culture

摘要: 以野生峨眉金线莲(Anoectochilus emeiensis K.Y.Lang.)的蒴果为外植体,采用正交设计进行组织培养的优化试验,以期建立峨眉金线莲种子组织培养体系。结果表明:外植体最佳消毒方案是75%酒精浸泡30 s,0.1% HgCl₂浸泡消毒15 min;峨眉金线莲最佳原初培养基为:MS+10%香蕉浸提液;最佳原球茎诱导和增殖培养基为:MS+10%香蕉+6-BA2 mg·L^-1+NAA0.5 mg·L^-1+活性炭(AC)3 g·L^-1;最佳生根壮苗培养基为1/2MS+10%香蕉+NAA 2 mg·L^-1+6-BA 0.5 mg·L^-1+活性炭1 g·L^-1。

Abstract: Culture in Vitro and propagation were carried out with capsules of Anoectochilus emeiensis by using orthogonal design with 4 levels of 5 factors.The result showed that the best explants disinfection method was to immerse 30 s in 75% alcohol plus 0.1%HgCl₂;the best medium for primary culture was MS+10% banana juice;MS+10% banana+6-BA 2 mg·L^-1+NAA 0.5 mg·L^-1+AC 3g·L^-1was the most suitable for bud induction and multiplication and the optimum medium for rooting and strong seedling culture was 1/2MS+10% banana+ NAA 2 mg·L^-1+6-BA 0.5 mg·L^-1+ AC 1g·L^-1.