Abstract: In this paper,SDS and nucleic acid adsorption column were used for extraction of the giant panda fecal DNA.According to the giant panda sex determination gene primers design factor,BDNF was taken as the positive control.Validation of the effectiveness of the primers was conducted for the use of fecal DNA from artificially reared giant pandas.It was showed that the results were reliable for the identification of the primers.Experiments showed that this method was convenient and reliable.