Abstract: In recent years,the growth environment of Cystoathyrium chinensis Ching has been seriously affected because of the destruction of human interference and geological disaster activities.The species is on the verge of extinction.For the protection and propagation of it,tissue culture propagation techniques were used to turn mature spores as explants.Researches were conducted on influences of spore germination by use of different sterilization,the medium and sucrose concentration.The results showed that the best sterilization was spores through 4 000 r·min^-1 centrifugal prepared spore suspension,0.1% mercuric chloride solution was centrifuged for 5 min,rinsed with sterile water for 4~5 times; the best germination medium was 1/2MS+20 g·L^-1 sucrose.After 90 days,the germination rate could reach 57.8%.