杉木优良无性系组培繁育技术研究

A study of Culture in Vitro and Propagation of Superior Clones of Cunninghamia lanceolata

摘要: 以杉木(Cunninghamia lanceolata Hook.)优良无性系3-3的基部萌条为外植体进行了组培快繁技术研究。试验结果表明:截干的最佳时间是在3月中旬,截干高度为30 cm,平均萌芽数达到47.2条;外植体最佳消毒方案是70%酒精浸泡15 s,后无菌水洗涤2次,再用0.1%升汞浸泡消毒6 min,无菌水洗涤6次;初代诱导最适基本培养基是MS培养基;增殖诱导最适培养基为MS+BA(0.5 mg·L^-1~0.7 mg·L^-1)+IBA(0 mg·L^-1~0.2 mg·L^-1);生根的最适培养基为1/2MS~1/4MS+0.5 mg·L^-1~1.0 mg·L^-1IBA+0.1 mg·L^-1NAA;杉木组培苗移栽的最适基质是泥炭土:黄心土:蛭石=4:2:1的混合土。

Abstract: Culture in Vitro and propagation were carried out by using basal sprouts of superior clones 3-3 Cunninghamia lanceolata as explants. The result showed that the optimal cutting trunk height was 30 cm in March and the average number of bud was 47.2; Best explants disinfection solution was to immerse 15s in 70% alcohol and use sterile water to wash twice and then to soak 6 min in 0.1% mercuric chloride and wash 6 times with sterile water; MS culture medium was the optimal basic culture in primary culture; Subculture medium was MS+BA(0.5 mg·L^-1~0.7 mg·L^-1)+IBA(0 mg·L^-1~0.2 mg·L^-1); Inducing-root medium was 1/2MS~1/4MS+0.5 mg·L^-1~1.0 mg·L^-1IBA+0.1 mg·L^-1NAA; The most suitable soil matrix proportion of transplanting was peat soil:yellow subsoil:vermiculite=4:2:1.