Abstract: In order to protect the resources of wild Cystoathyrium chinense,and explore the spore propagation technique,the experiment was conducted to observe the occurrence time and quantity of prothallium and sporophyte in 4 kinds medium(mixed media,sterile water,sterile habitat soil,and non-sterilized habitat soil) and 4 temperatures(10℃,15℃,20℃ and 25℃) by artificial controlling of light,water and other ecological factors.The result indicated that Cystoathyrium chinense spore germination was very strict to the requirement of the medium.The optimal medium for spore germination was non-sterilized habitat soil,while the quantity of prothallium was very few,and had longer germination time in others medium.This might be related to soil microorganisms and enzyme system.The most suitable temperature range was 15℃~20℃,especially at 20℃.The too high or too low temperature would inhibit the spore germination.It might be due to different structural characteristics of spores which had different requirements of light.