宜昌百合离体培养快繁技术研究

A Study of the Rapid Propagation Technology of Whiteflower Lily by the Isolated Culture

摘要: 以宜昌百合(Lilium leucanthum)的鳞片作为外植体进行离体组织培养,通过对宜昌百合的初代、继代和壮苗生根培养基的筛选进行研究,建立了较为适宜的宜昌百合组织培养快繁体系。其中:初代的最佳培养基为MS+1.5 mg·L^-1 6-BA+0.2 mg·L^-1 NAA;愈伤组织和芽的适宜增殖培养基为MS+1.0 mg·L^-1 6-BA+0.2 mg·L^-1 NAA;生根培养最佳培养基为1/2MS+IBA 0.5 mg·L^-1 。

Abstract: Whiteflower lily (Lilium leucanthum) scales were used as explants for tissue culture in vitro. Researches were conducted on the screening of whiteflower lily' primary culture medium,subculture medium and the medium of healthy seedling rooting in order to establish a suitable system of whiteflower lily tissue culture and rapid propagation. The results showed that the optimum medium of primary culture was MS + 1.5 mg·L^-1 6-BA + 0.2 mg·L^-1 NAA,the optimum proliferation medium for callus and buds was MS + 1.0 mg·L^-1 6-BA + 0.2 mg·L^-1 NAA. And the appropriate medium for rooting was 1/2MS + 0.5 mg·L^-1 IBA.