Abstract: The variability is rich among wild Dipsacus asperoides populations. The morphological characters of aboveground and underground parts form 9 major factors of character differences within wild populations. Aboveground parts,such as stem height,length of basal leafstalk,area and aspect ratio of basal leaf, the number and area of involucre of single plant. Underground parts include root weight,root number,content of active component in the dry,and so on. Regression analysis disdays a significant relationship in the characters of aboveground parts and root weight. It is better to choose the one with strong stem,long length of basal leafstalk,a long and narrow leaf,a big number and volume of involucre of single plant as high quality seeds to collect seeds. There is a strong correlation in morphological characters of wild Dipsacus asperoides populations. 9 wild populations were classified into 2 groups. One was Ge Sa-la Population,its content of active component in the dry was the highest,the other was Yong Xing Population,its root was the heaviest. Both populations could be used as high quality seeds.