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刘伟, 郭洁, 陈亚文. 4种蓝莓花粉量及花粉萌芽率的测定[J]. 四川林业科技, 2015, 36(2): 118-121,50. DOI: 10.16779/j.cnki.1003-5508.2015.02.028
引用本文: 刘伟, 郭洁, 陈亚文. 4种蓝莓花粉量及花粉萌芽率的测定[J]. 四川林业科技, 2015, 36(2): 118-121,50. DOI: 10.16779/j.cnki.1003-5508.2015.02.028
LIU Wei, GUO Jie, CHEN Ya-wen. Determination of the Pollen Number and Pollen Germination Rate of Four Blueberry Varieties[J]. Journal of Sichuan Forestry Science and Technology, 2015, 36(2): 118-121,50. DOI: 10.16779/j.cnki.1003-5508.2015.02.028
Citation: LIU Wei, GUO Jie, CHEN Ya-wen. Determination of the Pollen Number and Pollen Germination Rate of Four Blueberry Varieties[J]. Journal of Sichuan Forestry Science and Technology, 2015, 36(2): 118-121,50. DOI: 10.16779/j.cnki.1003-5508.2015.02.028

4种蓝莓花粉量及花粉萌芽率的测定

Determination of the Pollen Number and Pollen Germination Rate of Four Blueberry Varieties

  • 摘要: 以蓝莓4个品种花粉为试材,采用纤维素酶法和离体培养法,测定其花粉量,研究不同培养基上花粉萌芽率。结果表明:不同品种间花粉量存在差异,奥尼尔、夏普蓝、蓝玉一号、灿烂的平均花粉数分别为127、546、216、144粒/花药。培养基种类、温度对花粉萌芽率影响显著。适宜花粉萌芽的蔗糖浓度为15%;硼酸能够促进花粉萌芽,但是浓度过高时会抑制花粉萌芽,300μg·L-1硼酸浓度花粉萌芽率最高;25℃温度处理下,花粉萌发率较高。

     

    Abstract: In this paper,by using the fresh pollen from four blueberry varieties,studies were made of the pollen number and pollen germination rate of their flowers with the methods of cellulose and isolated culture medium. The results showed that the pollen numbers were different,the average of single anther from four varieties was respectively 127,546,216 and 144. The kinds of medium and the temperature significantly affected the pollen germination rate. The proper sucrose concentration for pollen germination of blueberry was 20% and the boric acid could promote the pollen germination. But if its concentration was too high,it would show an inhibition effect on the germination. The pollen germination was the highest when the boric acid concentration was 300 μg·L-1. The pollen germination rate was higher when the experiment was carried out at 25℃.

     

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