Abstract: In this paper,by using the fresh pollen from four blueberry varieties,studies were made of the pollen number and pollen germination rate of their flowers with the methods of cellulose and isolated culture medium. The results showed that the pollen numbers were different,the average of single anther from four varieties was respectively 127,546,216 and 144. The kinds of medium and the temperature significantly affected the pollen germination rate. The proper sucrose concentration for pollen germination of blueberry was 20% and the boric acid could promote the pollen germination. But if its concentration was too high,it would show an inhibition effect on the germination. The pollen germination was the highest when the boric acid concentration was 300 μg·L^-1. The pollen germination rate was higher when the experiment was carried out at 25℃.