Abstract: In June, 2010,20 months of seedlings and top branch and branch cuttings of Taxus yunnanensis were planted in the container in Pu'er seed breeding base, and the single factor was adopted to conduct randomized block design. In December 2011 the growth of tree height, ground diameter and collateral branches number were observed and analysed. The results showed that three treatments had significant difference in the tree height, diameter and collateral branches number. Treatment a (seedlings) displayed the best. Treatment C (branch cutting) showed the worst in the tree height and ground diameter. And treatment B (top branch cutting) had the worst collateral branching number.