Abstract: The determination and anaysis were made of the contents of macro-elements (N,P,K,Ca,Mg) and micro-elements (Fe,Zn) in organic tissue of 1 to 3 years old Eucalyptus grandis saplings and their biomass. The results showed that the contents of macro-elements in organic tissue were different, their ranking was bark,leaf > twig,root > stem,micro-elements were rich in leaf and root, and the accumulated amount of macro-elements in organic tissue of single tree was leaf > twig > bark > root or stem > stem or root,and that of macro-elements in 1 to 3 years old single tree was 12.45 g,136.19 g and 420.23 g, respectively,in which their yearly net accumulation was Ca > N > K > Mg > P,and the accumulation of Fe was much higher than Zn in single tree.