澳蜡花‘Snow Flake’的组织培养试验研究

An Experimental Study of Tissue Culture of Chamelaucium uncinatum ‘Snow Flake’

摘要: 澳蜡花(Chamelaucium uncinatum)‘Snow Flake’作为新一代木本切花,具有较高的观花、观叶及经济价值。本试验通过对澳蜡花‘Snow Flake’组培技术的研究,为其进行工厂化繁育奠定基础。结果表明,用0.1% HgCl2进行外植体消毒最佳时间为7 min;MS1+6-BA1.0 mg·L^-1+IBA0.2 mg·L^-1+KT0.5 mg·L^-1为最佳初代培养基;最适继代培养基为WPM1+6-BA 0.5 mg·L^-1+IBA 0.2 mg·L^-1+GA3 2.5 mg·L^-1,增殖系数4.2,增殖倍数3倍~6倍;最适生根培养基为1/2MS2+IBA0.2 mg·L^-1+0.1 mg·L^-1 GGR。

Abstract: As a new generation of woody cut flowers, C. uncinatum ‘Snow Flake’ has a high ornamental and economic value. Based on the study of tissue culture of ‘Snow Flake’,the aim of this experiment was to lay the foundation for Industrial production of ‘Snow Flake’. The results showed that 7 minutes were the best time to sterilize explants by using 0.1% HgCl2. MS1 + 6-BA1.0 mg·L^-1 + IBA0.2 mg·L^-1 + KT0.5 mg·L^-1 was the optimum primary culture medium, the best subcultuer medium was WPM1 + 6-BA 1.0 mg·L^-1 + IBA 0.2 mg·L^-1 + GA3 1.5 mg·L^-1,multiplication coefficient was 4.2,multiplication was 3~6. The most suitable rooting medium was 1/2MS2 + IBA0.2 mg·L^-1 + 0.1 mg·L^-1 GGR.