Abstract: Studies were made of the seedling cultivation techniques of Nyssa yunnanensis and the growth characteristics of this species during the seedling period, including researches on seed collection, seed treatment, seedling raising, and growth observation during the seedling stage. The results showed that the seed of Nyssa yunnanensis did not have dormancy stage, the seeding work was suitable to be done right after the collection of seeds. The seeds began germinating one week after seeding, the seedling emergence occurred 25 days after seeding, and the proper way of storing seeds was to be mixed with sand. The seven month old seedlings could attain the size suitable for field planting, within one year after seeding, the seedlings showed the growth process of ‘slowness-quickness-slowness’, the growth peak of the seedling occurred from June to July, and within this fast-growing period, sufficient water and fertilizer should be applied to promote the growth of the seedlings.