Abstract: In this paper, by adopting the single-factor and orthogonal experiments the researches were conducted on the fermentation medium of Bacillus licheniformis YB15, and the results showed that its optimum components were glucose 5.0 g, yeast extract 7.5 g, peptone 7.5 g and ammonium sulphate 5.0 g. On this basis, its optimum fermentation conditions were also studied, which were temperature at 25℃, pH 6.5, culture broth quantity 100 mL·300 mL^-1 flask and inoculation quantity of 4 mL. The result of the growth curve of Bacillus licheniformis showed that 12~18 hours were the best time to collect it.