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邢慧子, 高平, 邓小宽, 梁歌, 李旭廷, 王斌, 李思聪, 张敏. 细毡毛忍冬叶提取物的抗菌、抗氧化活性研究[J]. 四川林业科技. DOI: 10.12172/202403190001
引用本文: 邢慧子, 高平, 邓小宽, 梁歌, 李旭廷, 王斌, 李思聪, 张敏. 细毡毛忍冬叶提取物的抗菌、抗氧化活性研究[J]. 四川林业科技. DOI: 10.12172/202403190001
XING HuiZi, GAO Ping, DENG Xiaokuan, LIANG Ge, LI Xuting, WANG Bin, LI Sicong, ZHANG Min. Antibacterial and Antioxidant Activity of The Leaves of Lonicera Similis Hemsl.[J]. Journal of Sichuan Forestry Science and Technology. DOI: 10.12172/202403190001
Citation: XING HuiZi, GAO Ping, DENG Xiaokuan, LIANG Ge, LI Xuting, WANG Bin, LI Sicong, ZHANG Min. Antibacterial and Antioxidant Activity of The Leaves of Lonicera Similis Hemsl.[J]. Journal of Sichuan Forestry Science and Technology. DOI: 10.12172/202403190001

细毡毛忍冬叶提取物的抗菌、抗氧化活性研究

Antibacterial and Antioxidant Activity of The Leaves of Lonicera Similis Hemsl.

  • 摘要: 为代替金银花花蕾作为饲料添加剂,降低饲料成本,充分利用细毡毛忍冬叶资源,因此探究细毡毛忍冬叶粗提物、工艺优化提取物及精制提取物的抗菌、抗氧化活性。测定细毡毛忍冬叶不同提取物的主要活性成分含量,并进行各提取物对大肠杆菌、金黄色葡萄球菌、鼠寒沙门氏菌及铜绿假单胞菌的体外抑菌实验,并对抑菌效果较好的细毡毛忍冬叶精制物进行MIC(最小抑菌浓度)及MBC(最低杀菌浓度)测定。使用ABTA、DPPH、FRAP三种抗氧化能力检测方法测定各提取物总抗氧化能力。(1)细毡毛忍冬叶精制产物在抑菌活性实验中表现出显著抑菌活性,抑菌能力与金银花花蕾精制产物相近,比粗提物及工艺优化产物高。(2)细毡毛忍冬叶精制物对大肠杆菌、金黄色葡萄球菌、鼠寒沙门氏菌、铜绿假单胞菌的MIC分别为12.5 mg/mL、6.25 mg/mL、1.56 mg/mL、3.125 mg/mL , MBC分别为25 mg/mL、12.5 mg/mL、3.125 mg/mL、12.5 mg/mL。(3)细毡毛忍冬叶精制物具有较强抗氧化性,其抗氧化能力随着溶液浓度的增加而逐步升高,且抗氧化性高于粗提物、工艺优化产物及金银花精制物,表明经过富集纯化的细毡毛忍冬叶产物的抗氧化性显著增加。细毡毛忍冬叶精制产物具有较好的抗菌、抗氧化活性,具有代替金银花作为饲料添加的潜力。

     

    Abstract: In order to replace Lonicera japonica Thunb. bud as feed additive, reduce feed cost and make full use of the leaves of Lonicera similis Hemsl. resources, the antibacterial and antioxidant activities of crude extract, process optimization extract and refined extract of the leaves of L.similis Hemsl. were investigated. The main active ingredients of different extracts of the leaves of L.similis Hemsl. were determined, and the in vitro antibacterial experiments of each compound on Escherichia coli, Staphylococcus aureus, Salmonella typhimurium and Pseudomonas aeruginosa were carried out, and the MIC and MBC experiments were carried out on the refined products with better antibacterial effect. The total antioxidant capacity of each extract was determined by ABTA, DPPH and FRAP. (1) The refined products of The leaves of L.similis Hemsl. showed significant antibacterial activity in the antibacterial activity experiment, and the antibacterial ability was similar to that of the flower buds of Lonicera japonica Thunb., and higher than that of the crude extract and optimized products. (2) The MIC of the leaves of L.similis Hemsl. refined products on Escherichia coli, Staphylococcus aureus, Salmonella typhimurium and Pseudomonas aeruginosa were 12.5 mg/mL, 6.25 mg/mL, 1.56 mg/mL and 3.125 mg/mL, respectively, and the MBC were 25 mg/mL, 12.5 mg/mL, 3.125 mg/mL and 12.5 mg/mL. (3) The leaves of L.similis Hemsl. exhibited strong antioxidant activity, and its antioxidant capacity gradually increased with the increase of solution concentration, and was higher than that of crude extract, process optimization product and Lonicera japonica Thunb. extract, indicating that the antioxidant capacity of L.similis Hemsl. after enrichment and purification was significantly increased. The leaves of L.similis Hemsl. had good antibacterial and antioxidant activities.

     

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