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李健威, 崔鑫月, 蒋清松, 等. 大熊猫主食竹叶围微生物抗生素抗性基因[J/OL]. 四川林业科技, 2024, 45[2024-05-16]. DOI: 10.12172/202312260003
引用本文: 李健威, 崔鑫月, 蒋清松, 等. 大熊猫主食竹叶围微生物抗生素抗性基因[J/OL]. 四川林业科技, 2024, 45[2024-05-16]. DOI: 10.12172/202312260003
LI J W, CUI X Y, JIANG Q S, et al. Antibiotic resistance genes (ARGs) in phyllosphere bacteria of giant panda’s staple food bamboo[J/OL]. Journal of Sichuan Forestry Science and Technology, 2024, 45[2024-05-16]. DOI: 10.12172/202312260003
Citation: LI J W, CUI X Y, JIANG Q S, et al. Antibiotic resistance genes (ARGs) in phyllosphere bacteria of giant panda’s staple food bamboo[J/OL]. Journal of Sichuan Forestry Science and Technology, 2024, 45[2024-05-16]. DOI: 10.12172/202312260003

大熊猫主食竹叶围微生物抗生素抗性基因

Antibiotic resistance genes (ARGs) in phyllosphere bacteria of giant panda’s staple food bamboo

  • 摘要: 抗菌药物在为人类服务的同时,使其他生物体产生新的抗生素抗性基因(Antibiotic resistance genes,ARGs),严重危害人类和动物的健康。ARGs分布范围广泛,草食性动物主要通过采食而接触存在ARGs的植物微生物。大熊猫(Ailuropoda melanoleuca)在进化过程中食性特化成以竹子为主食。研究在四川栗子坪国家级自然保护区展开,着眼于大熊猫主食竹叶围微生物,采用宏基因组测序,探讨大熊猫食物资源微生物ARGs的组成和多样性。分析发现大熊猫主食竹叶围细菌ARGs在Class水平上有抗四环素类(Tetracycline)、抗大环内酯-林可酰胺-链霉素类(MLS)、抗糖肽类(Glycopeptide)、抗多肽类(Peptide)等。峨热竹(Arundinaria spanostachya)叶围细菌ARGs的Sobs指数显著大于丰实箭竹(Fargesia ferax);而Shannon指数在三种大熊猫主食竹间没有显著差异。然后通过NMDS分析发现三种大熊猫主食竹间叶围细菌ARGs组成结构间具有明显差异。经过LEfSe寻找区别三种大熊猫主食竹细菌ARGs的重要种类,峨热竹中Glycopeptide、Phenicol和Bicyclomycin有最大作用;丰实箭竹中Aminocoumarin和Pleuromutilin有最大作用。Glycopeptide和Phenicol主要由PseudomonasSphingomonas携带,而Aminocoumarin和Pleuromutilin主要由SphingomonasLichenibacterium携带等。研究提示了大熊猫采食的竹叶叶围微生物含有丰富的ARGs,在日常监测中需重点监测在携带区分三种大熊猫主食竹叶围细菌ARGs的细菌类群(如假单胞菌属、鞘脂单胞菌属等);此外,还需关注其它环境(土壤、溪流、乔木等)微生物ARGs的状况,以期全面了解环境微生物及其携带的ARGs对大熊猫的潜在影响。

     

    Abstract: Antibiotics not only serve humans, but also generate new antibiotic resistance genes (ARGs) in other organisms, seriously endangering the health of humans and animals. ARGs are widely distributed, and herbivorous animals mainly come into contact with plant microorganisms’ ARGs through feeding. The giant panda (Ailuropoda melanoleuca) has specialized its diet to rely on bamboo as its staple food. The study was conducted in Sichuan Liziping National Nature Reserve Administration, focuses on the composition and diversity of microbial ARGs in the food resources of giant pandas, using metagenomic sequencing. After analysis, it was found that the ARGs of giant panda’s staple food bamboo phyllosphere bacteria mainly include Tetracycline, MLS (macrolide lincomycin streptomycin), Glycopeptides, Peptides, etc. at the class level. The Sobs index of ARGs around the leaves of Arundinaria spanostachya is significantly higher than that of Fargesia ferax. However, there was no significant difference in the Shannon index among the three bamboo species. Further analysis by NMDS revealed significant differences in the composition and structure of ARGs among the three main food sources of giant pandas. Through LEfSe analysis, important species of phyllosphere bacteria ARGs were identified to distinguish the three main food sources of giant pandas. Glycopeptide, Phenol, and Bicyclomycin were found to have the greatest effect in Arundinaria spanostachya; Aminocoumarin and Pleurocutilin have the greatest effects in the Fargesia ferax. Glycopeptide and Phenol mainly existed in Pseudomonas, Sphingomonas, etc., while Aminocoumarin and Pleurocutilin mainly existed in Sphingomonas, Lichenibacterium, etc. This study suggests that the bamboo phyllosphere microbiota consumed by giant pandas contain abundant ARGs. And in daily monitoring, it is necessary to focus on monitoring the most important bacterial groups (such as Pseudomonas, Sphingomonas, etc.), which are distinguishing the antibiotic resistance genes among the three giant panda’s staple food bamboo phyllosphere bacteria. In addition, attention should also be paid to the status of microbial ARGs in other environments (soil, streams, trees, etc.), in order to comprehensively understand the potential impact of environmental microorganisms and their antibiotic resistance genes on giant pandas.

     

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