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刘培琴, 姜丽琼, 卢麒宇, 等. 白及叶枯病病原菌分离鉴定与致病性测定[J]. 四川林业科技, 2024, 45(3): 142−146. DOI: 10.12172/202311140002
引用本文: 刘培琴, 姜丽琼, 卢麒宇, 等. 白及叶枯病病原菌分离鉴定与致病性测定[J]. 四川林业科技, 2024, 45(3): 142−146. DOI: 10.12172/202311140002
LIU P Q, JIANG L Q, LU Q Y, et al. Isolation, identification and pathogenicity determination of pathogen of Bletilla Striata leaf blight[J]. Journal of Sichuan Forestry Science and Technology, 2024, 45(3): 142−146. DOI: 10.12172/202311140002
Citation: LIU P Q, JIANG L Q, LU Q Y, et al. Isolation, identification and pathogenicity determination of pathogen of Bletilla Striata leaf blight[J]. Journal of Sichuan Forestry Science and Technology, 2024, 45(3): 142−146. DOI: 10.12172/202311140002

白及叶枯病病原菌分离鉴定与致病性测定

Isolation, identification and pathogenicity determination of pathogen of Bletilla Striata leaf blight

  • 摘要: 为明确白及叶枯病病原菌种类,对白及叶枯病病原进行分离、纯化及rDNA-ITS序列分析鉴定其种属,并将纯化病原菌回接于白及植株,评价其致病性。结果表明,从白及患病样品上共分离6株真菌,其中有三株镰刀菌,分别为角化镰刀菌(Fusarium keratoplasticum )、尖孢镰刀菌(Fusarium oxysporum)、木贼镰刀菌(Fusarium equiseti);一株曲霉菌,为日本曲霉(Aspergillus japonicus);一株灰霉,为灰葡萄孢霉(Botrytis cinerea);一株毛霉,为易脆毛霉(Mucor fragilis)。经致病性测定,发现上述6株真菌对白及均有致病性。

     

    Abstract: In order to clarify the pathogenic bacteria species of Bletilla Striata leaf blight, the pathogen of B. Striata leaf blight was isolated, purified and identified by rDNA-ITS sequence analysis, the purified pathogen was grafted to B. Striata plants to evaluate its pathogenicity. The results showed that a total of 6 fungi strains were isolated from the diseased samples of B. striata, including three strains of Fusarium, namely: F. keratoplasticum, F. oxysporum and F. equiseti; a strain of Aspergillus japonicus; a strain of Botrytis cinerea; a strain of Mucor fragilis. According to the pathogenicity test, it was found that the above 6 strains of fungi were all pathogenic to B. Striata.

     

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