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胡竞月, 梅小平, 吴斌, 等. GC-MS/SIM法同时测定山苍子精油中主要成分的绝对含量[J/OL]. 四川林业科技, 2024, 45[2024-03-05]. DOI: 10.12172/202311090001
引用本文: 胡竞月, 梅小平, 吴斌, 等. GC-MS/SIM法同时测定山苍子精油中主要成分的绝对含量[J/OL]. 四川林业科技, 2024, 45[2024-03-05]. DOI: 10.12172/202311090001
HU J Y, MEI X P, WU B, et al. Simultaneous determination of the absolute contents of major components in Litsea cubeba essential oil by GC-MS/SIM[J/OL]. Journal of Sichuan Forestry Science and Technology, 2024, 45[2024-03-05]. DOI: 10.12172/202311090001
Citation: HU J Y, MEI X P, WU B, et al. Simultaneous determination of the absolute contents of major components in Litsea cubeba essential oil by GC-MS/SIM[J/OL]. Journal of Sichuan Forestry Science and Technology, 2024, 45[2024-03-05]. DOI: 10.12172/202311090001

GC-MS/SIM法同时测定山苍子精油中主要成分的绝对含量

Simultaneous determination of the absolute contents of major components in Litsea cubeba essential oil by GC-MS/SIM

  • 摘要: 为了探究山苍子精油中主要成分的绝对含量,建立了一种气相色谱-质谱/选择离子扫描(gas chromatography mass spectrometry/selective ion monitoring,GC-MS/SIM)方法,对其中主要成分(α-蒎烯、β-蒎烯、D-柠檬烯、1,8-桉叶素、芳樟醇、香茅醛、柠檬醛)进行了定性和定量分析。该方法简便、高效,在25 min内山苍子精油中各成分分离良好。各成分在一定范围内均线性关系良好( R2> 0.999),精密度、稳定性及重复性均符合要求,平均加标回收率为93.40%~107.35% 。结果表明:该方法符合方法学验证要求,可用于山苍子精油的多成分含量测定,并为其质量评价提供依据。

     

    Abstract: In order to investigate the absolute contents of the main components in Litsea cubeba essential oil, a gas chromatography mass spectrometry/selective ion monitoring (GC-MS/SIM) was established for the qualitative and quantitative analysis of the main components (α--pinene, β--pinene, D-limonene, 1, 8-cineole, linalool, citronellal, citral) were analyzed qualitatively and quantitatively. The method is simple and efficient, and the components in Litsea cubeba essential oil were well separated within 25 min. The linearity of the method was good over a certain range (R2> 0.999), and the precision, stability and reproducibility were all in accordance with the requirements, with the average recoveries of 93.40%-107.35%. The results showed that the method meets the requirements of methodological validation, and can be used for the determination of the multi-component content of Litsea cubeba essential oil and provide a basis for its quality evaluation.

     

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