Abstract: In order to investigate the absolute content of the main components in Litsea cubeba essential oil, a gas chromatography mass spectrometry/selective ion monitoring (GC-MS/SIM) was established for the qualitative and quantitative analysis of the main components (α-pinene, β-pinene, D-limonene, 1, 8-cineole, linalool, citronellal, citral) were analyzed qualitatively and quantitatively. The method is simple and efficient, and the components in Litsea cubeba essential oil can be separated well within 25 min. The linearity of the method was good over a certain range (R²> 0.999), and the precision, stability and reproducibility were all in accordance with the requirements, with the average recoveries of 93.40%-107.35%. The results showed that the method meets the requirements of methodological validation, and can be used for the determination of the multi-component content of Litsea cubeba essential oil and provide a basis for its quality evaluation.