Abstract: By analyzing the changes of ecosystem service value per unit area during the primary succession of the Hailuogou glacier retreat area, it could provide some help for the policies and guidelines of positive effects of artificial ecosystem management, reconstruction and restoration. Six sample plots (12, 30, 40, 50, 70 and 120 years) were set up in Hailuogou glacier retreat site. According to the Specification for Assessment of Forest Ecosystem Service Functions (LY/T 1721-2008), the ecosystem service values of different sites were calculated in five aspects: nutrient element accumulation, carbon sequestration and oxygen release, soil conservation, water retention and species diversity per unit area. The results were as follows: (1) The ecosystem service values reached the maximum value of 23721.95 Yuan·hm⁻²·a⁻¹ in sample plot 5 and the minimum value of 7326.49 Yuan·hm⁻²·a⁻¹ in sample plot 1, and the growth rate of ecosystem service values per unit area showed a process of rapid increase at first and then slow decrease. (2) The maximum growth rate of ecosystem service values per unit area was +8.22% per year from plot 1 to plot 2, and the minimum growth rate was −1.64% per year from plot 2 to plot 3. (3) The ecosystem service functions in different sites were mainly carbon sequestration and oxygen release, water conservation and biodiversity. It was found that the main ecosystem service functions in the Hailuogou glacier retreat were basically the same as those in other forest ecosystems, and the ecosystem service values per unit area varies significantly among different sites during the succession process.