Abstract: In this experiment, the effects of spike type, substrate type and hormone concentration on the cuttage propagation of Machilus nanmu were analyzed by orthogonal experiment. Through variance analysis and mean value comparison, the optimum cutting combination of Machilus nanmu was screened out in order to provide a theoretical basis for the large-scale propagation of Machilus nanmu. The results indicated that panicle type and hormone concentration had significant effects on rooting rate of Machilus nanmu cutting, but had no significant effects rooting number and the length of taproots. The substrate types had significant effects on rooting rate and length of taproots, but had no significant effects on rooting number. Through further screening, it was found that the rooting rate of the shoots with perlite: vermiculite = 1:4 was the highest when soaked in ABT concentration of 100 mg·L⁻¹ solution for 2 h. When the rooting rate was high, the taproot length was the longest when the twigs of vermiculite were soaked in the ABT concentration of 100 mg·L⁻¹ solution for 2 h, and the rooting effect was ideal.